Anophelinae mosquitoes are vectors of human malaria, a disease that infects hundreds of millions of people and causes almost 600,000 fatalities annually. Despite their medical importance, laboratory studies on key aspects of Anophelinae reproductive biology have been limited, and in particular, relatively little is known about the role of juvenile hormone (JH) in the control of female reproduction. The study presented here attempts to fill a gap of knowledge in our understanding of the JH control of ovarian development in female Anophelinae mosquitoes, using Anopheles albimanus as a model. Our studies revealed that JH controls the tempo of maturation of primary follicles in An. albimanus in a similar manner to that previously described in Aedes aegypti. At adult eclosion JH hemolymph titer was low, increased in 1-day old sugar-fed insects, and decreased in blood fed individuals. JH titers decreased if An. albimanus females were starved, and were reduced if insects emerged with low teneral reserves, precluding previtellogenic ovarian development. However, absolute hemolymph titers were lower than Ae. aegypti. Decapitation experiments suggested that if teneral reserves are sufficient, factors from the head activate JH synthesis by the corpora allata (CA) during the first 9–12 h after adult emergence. In conclusion, our studies support the hypothesis that JH controls previtellogenic ovarian development in female An. albimanus mosquitoes, in a similar manner that have been described in Culicinae.

嗜人按蚊是人类疟疾的媒介，这种疾病感染数亿人，每年造成近60万人死亡。尽管有医学上的重要性，但对按蚊生殖生物学关键方面的实验室研究却很有限，尤其是，关于幼年激素（JH）在控制女性生殖中的作用的了解相对较少。这里提出的研究试图以白带按蚊为模型，来填补我们对雌性按蚊的卵巢发育的JH控制的了解的知识空白。我们的研究表明，JH控制着An。初级卵泡的成熟速度。albimanus以类似的方式与先前所描述的埃及伊蚊。在成年雏鸡中，JH的血淋巴滴度很低，在1天龄的食糖昆虫中增加，而在用血液喂养的个体中则下降。如果An， JH效价降低。albimanus雌性被饿死，如果昆虫出现了较低的矿物储备，则将其减少，这排除了前玻璃体形成卵巢的发育。但是，绝对血淋巴滴度低于Ae。伊蚊。斩首实验表明，如果探矿权储备充足，则成年后的头9–12小时内，头部的因子会激活体集（CA）的JH合成。总之，我们的研究支持JH控制女性An的玻璃体前卵巢发育的假说。白bi 蚊，以类似的方式在蚊属中进行了描述。