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“  The Growth Acceleration  of Microorganism  In Yoghurt  by the Extract of Asparagus ”
Introduction ,[object Object]
[object Object],[object Object],[object Object],Purpose
[object Object],[object Object],[object Object],[object Object],Hypothesis
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Variable
[object Object],[object Object],[object Object],[object Object],[object Object],Asparagus  ( Asparagus officinalis ) Asparagus http :// www . ics . uci . edu / ~eppstein / pix / tanaka2 / Asparagus - m . jpg
Inulin  falls under the general class of carbohydrates called fructans, those polymers containing fructose  The natural biosynthesis of  inulin  within plant cells begins by the cells using a vacuolar enzyme, sucrosesucrose fructosyltransferase  ( SST ) , to catalyze the reaction.    SST Equation   :   GF + GF  GFF + G Inulin
Figure 1 :  Chemical Structure of sucrose  ( left ) ,  inulin ( center ) , oligofructose  ( right )   Inulin
[object Object],[object Object],[object Object],[object Object],Properties of Inulin
Figure 2  :   Pathogenic microflora suppression, from Roberfroid et al .  1998 and Rolfe 1981   Property of Inulin
[object Object],[object Object],[object Object],[object Object],Experimental groups Materials and methods
Extracting the substance from asparagus Figure   3 :   Inulin  production process   Materials and methods Raw asparagus  Purified asparagus
Materials and methods Cleaned asparagus
Materials and methods Crushed asparagus
Materials and methods Evaporator
Materials and methods Exact of asparagus
Making yoghurt for experiment Materials and methods 1.  Heat the milk to boiling point and  Continue simmering on a low gas for about 5-10 minutes
Materials and methods 2.  Pour the milk into the glass jar
Materials and methods 3.  Add asparagus extracting solution  1 ml in 10 bottles, 2.5 ml in 10 bottles and 5 ml in 10 bottles
Materials and methods 4.  Add 5 ml of natural yoghurt to the milk in the jar and stir the mixture
Materials and methods 5.  Incubate in the oven at temperature 44°C
[object Object],[object Object],[object Object],Testing pH Materials and methods
[object Object],[object Object],[object Object],[object Object],Finding the quantity of lactic acid Materials and methods
Chart (Line) :  Changes of pH in Yoghurt fermentation at 0-6 hours Result Δ
Chart (column) :  Changes of pH in Yoghurt fermentation at 0-6 hours Result
Chart (Line) :  Changes of lactic acid in Yoghurt fermentation  at 0-6 hours Result
Chart (column) :  Changes of lactic in Yoghurt fermentation at 0-6 hours Result
Conclusion The study of making yoghurt by adding 1 mg/ml, 2.5 mg/ml and 5 mg/ml   of the asparagus extract into  yoghurt was checked the result at the 0, 1 st ,2 nd ,3 rd ,4 th ,5 th  and 6 th   hours.   From the experiment,  Δ pH of the controlled group,  1 mg/ml, 2.5 mg/ml and 5 mg/ml are 2.002, 2.057, 2.043 and 1.895, respectively. Besides, the amount of lactic acid from the  controlled group,  1 mg/ml, 2.5 mg/ml and 5 mg/ml are 0.0815 mol, 0.09 mol, 0.087 mol and 0.0755 mol, respectively.  So, 1 mg/ml  of asparagus extract is best accelerate the growth of  microorganism in yoghurt that have an effect on the decreasing period of fermentation.
[object Object],[object Object],[object Object],[object Object],Reference
[object Object],[object Object],[object Object],[object Object],[object Object],Acknowledgement

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Inulin

  • 1. “ The Growth Acceleration of Microorganism In Yoghurt by the Extract of Asparagus ”
  • 2.
  • 3.
  • 4.
  • 5.
  • 6.
  • 7. Inulin falls under the general class of carbohydrates called fructans, those polymers containing fructose The natural biosynthesis of inulin within plant cells begins by the cells using a vacuolar enzyme, sucrosesucrose fructosyltransferase ( SST ) , to catalyze the reaction. SST Equation : GF + GF GFF + G Inulin
  • 8. Figure 1 : Chemical Structure of sucrose ( left ) , inulin ( center ) , oligofructose ( right ) Inulin
  • 9.
  • 10. Figure 2 : Pathogenic microflora suppression, from Roberfroid et al . 1998 and Rolfe 1981 Property of Inulin
  • 11.
  • 12. Extracting the substance from asparagus Figure 3 : Inulin production process Materials and methods Raw asparagus Purified asparagus
  • 13. Materials and methods Cleaned asparagus
  • 14. Materials and methods Crushed asparagus
  • 15. Materials and methods Evaporator
  • 16. Materials and methods Exact of asparagus
  • 17. Making yoghurt for experiment Materials and methods 1. Heat the milk to boiling point and Continue simmering on a low gas for about 5-10 minutes
  • 18. Materials and methods 2. Pour the milk into the glass jar
  • 19. Materials and methods 3. Add asparagus extracting solution 1 ml in 10 bottles, 2.5 ml in 10 bottles and 5 ml in 10 bottles
  • 20. Materials and methods 4. Add 5 ml of natural yoghurt to the milk in the jar and stir the mixture
  • 21. Materials and methods 5. Incubate in the oven at temperature 44°C
  • 22.
  • 23.
  • 24. Chart (Line) : Changes of pH in Yoghurt fermentation at 0-6 hours Result Δ
  • 25. Chart (column) : Changes of pH in Yoghurt fermentation at 0-6 hours Result
  • 26. Chart (Line) : Changes of lactic acid in Yoghurt fermentation at 0-6 hours Result
  • 27. Chart (column) : Changes of lactic in Yoghurt fermentation at 0-6 hours Result
  • 28. Conclusion The study of making yoghurt by adding 1 mg/ml, 2.5 mg/ml and 5 mg/ml of the asparagus extract into yoghurt was checked the result at the 0, 1 st ,2 nd ,3 rd ,4 th ,5 th and 6 th hours. From the experiment, Δ pH of the controlled group, 1 mg/ml, 2.5 mg/ml and 5 mg/ml are 2.002, 2.057, 2.043 and 1.895, respectively. Besides, the amount of lactic acid from the controlled group, 1 mg/ml, 2.5 mg/ml and 5 mg/ml are 0.0815 mol, 0.09 mol, 0.087 mol and 0.0755 mol, respectively. So, 1 mg/ml of asparagus extract is best accelerate the growth of microorganism in yoghurt that have an effect on the decreasing period of fermentation.
  • 29.
  • 30.