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2020
Objectives: We and others have shown that Aspilia pluriseta is associated with various biological activities. However, there is a lack of information on A. pluriseta cytotoxicity. This has created an information gap about the safety of A. pluriseta extracts. As an extension to our recent publication on the antimicrobial activity and the phytochemicals characterization of A. pluriseta root extracts, here we report the missing data on cytotoxicity of tested extracts. We evaluated the potential cytotoxicity of the root extracts on Vero cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: We show that all solvent extracts (except methanolic solvent fractions) had cytotoxic concentration values that killed 50% of the Vero cells (CC50) greater than 20 µg/mL and selectivity index (SI) of greater than 1.0. Taken together, we demonstrate that, A. pluriseta extract fractions’ earlier reported bioactivity are within the acceptable cytotoxicity and se...
Aspilia pluriseta is associated with various bioactivities, although with limited scientific justification. In this study, we evaluated the antimicrobial activity, and characterized the phytochemicals of root extracts of A. pluriseta aimed at validating its therapeutic potential. We used BACTEC MGIT™ 960 system to test for antitubercular activity, disc-diffusion together with the microdilution method to evaluate antimicrobial activities and qualitative phytochemical tests together with gas chromatography-mass spectrometry (GC-MS) analysis to determine the phytochemicals that associated with A. pluriseta extracts activity. We show that methanolic crude extract (at 1 g/mL) had high Mycobacterium tuberculosis (MTB) inhibitory activity (0 growth unit) and considerable potency against Escherichia coli (11.7 mm), Staphylococcus aureus (9.0 mm), and Candida albicans (7.7 mm). All the extract fractions exerted remarkable antimycobacterial activities with minimum inhibitory activity of between 6.26 - 25 μg/mL. The highest antimicrobial activity of petroleum ether and dichloromethane fraction was against E. coli at inhibition zone diameters of 8.3 mm, and 8.0 mm, respectively, while ethyl acetate fraction was against S. aureus with an inhibition zone of 8.7 mm. Methanolic fraction exhibited broad-spectrum activity against 87.5% of the tested microbes (inhibition zones 6.3-8.3 mm). Furthermore, we qualitatively detected terpenoids, alkaloids, and phenolics such as flavonoids, and anthraquinones in extract fractions. GC-MS analysis detected an abundance of fatty acid esters, 2-hydroxy-1-(hydroxymethyl) ethyl ester-hexadecanoic acid, and 2,3-dihydroxy propyl ester-octadecanoic acid and four alkanes. Taken together, we show that A. pluriseta extract fractions (especially ethyl acetate and methanolic fractions) have strong selective antitubercular activity, and thus, we scientifically validate the use of A. pluriseta as a potential source for the discovery of novel antitubercular agents.
Journal of Medicinal Plants Research
In vitro antioxidant and cytotoxic activity of the root extract of Aspilia mossambicensis (Oliv) Wild (Asteraceae)2020 •
Aspiliamossambicensis(Oliv) Wild is used by Traditional Health Practitioners in northeastern Tanzania, for treatment of cancers. In order to evaluate these claims root powder of the plant was extracted with dichloromethane: methanol (1:1), followed by vacuum liquid chromatography fractionation to obtain dichloromethane, ethyl acetate and methanol fractions which were screened for brine shrimp toxicity and antioxidant activity using DPPH and FRAP assays. The ethyl acetate fraction exhibited higher toxicity on brine shrimp larvae (LC50 = 12.87 µg/ml) than cyclophosphamide (LC50 = 16.12 µg/ml), and antioxidant activity with an EC50 of 200 µg/ml for DPPH and 53.92 μM ECGC equivalent/g dry weight for FRAP assay. The ethyl acetate fraction was cytotoxic against HeLa cancer cells (IC50 50.77 ± 1.69 µg/ml), causing cell cycle arrest at the M phase, phosphatidylserine (PS) externalization and activation of caspase 3 and 8. Four compounds were isolated from this fraction; (-)-Angeloylgrandifl...
The uses of medicinal plants for treatment of various infections in traditional communities have been an age-long practice. This provides the rationale to study medicinal plant extracts as a possible source of alternative therapy against infections. The current study was undertaken to evaluate the phytochemical and antimicrobial properties of Aspilia africana. The antimicrobial activity and minimum inhibitory concentration (MIC) of the extracts of A. africana were evaluated against eight organisms-Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Candida albicans, Aspergillus niger, Penicillum spp and Fusarium spp. The ethanolic and aqueous extracts were obtained by standard methods. Antimicrobial activity was conducted using a modified agar well diffusion method. The phytochemical screening and analysis carried out in this study showed that the plant extracts contains alkaloids (6.350±0.84), saponins (2.260±0.15), flavonoids (2.006±0.11), tannins (0.881±0.04) and phenols (0.109±0.02). The result showed that ethanolic extract of A. africana exerted antimicrobial effect on the test organisms at 25 mg/ml, 50 mg/ml and 100 mg/ml concentrations, while the hot aqueous extract exerted Original Research Article Ezeigbo et al.; EJMP, 15(2): 1-6, 2016; Article no.EJMP.26481 2 antimicrobial effect at 100 mg/ml only on Staphylococcus aureus and Pseudomonas aeruginosa. The ethanolic extract of A. Africana showed the highest antimicrobial activity with diameter of zone of inhibition of 3.35 mm to 17.9 mm at 100 mg/concentration. The minimum inhibitory concentration (MIC) of the ethanolic extracts was at a concentration of 25 mg/ml. The antimicrobial activity of the extract could be enhanced if the components are purified. This plant therefore holds a promising potential source of new drug for treating infections caused by these clinical pathogens.
2013 •
Plants
Selective Anticancer Properties, Proapoptotic and Antibacterial Potential of Three Asplenium Species2021 •
The ferns Asplenium ceterach L., Asplenium scolopendrium L. and Asplenium trichomanes L. have wide application in traditional medicine worldwide. However, the scientific research on their anticancer and antibacterial properties is insufficient. The present article aims to provide more information on this topic. Extracts derived from the aerial parts of A. ceterach, A. scolopendrium and A. trichomanes were examined using a panel of in vitro assays with different bacterial and mammalian cells. The cytotoxicity and anticancer activity of the samples were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Trypan blue assays with three human (A549, FL, HeLa) and three murine (3T3, TIB-71, LS48) cell lines. Inhibitory effects on the growth of Gram-positive (Bacillus cereus) and Gram-negative (Pseudomonas aeruginosa) bacteria were determined by the agar diffusion assay. Apoptosis-inducing properties of the extracts were analyzed by flow cytometry. Superoxide...
Advances in Life Science and Technology
Antimicrobial activity and Chemical Composition of the flowers of Aspilia africana2014 •
The African Journal of Pharmacology and Therapeutics
Antimicrobial activity of compounds isolated from the leaves of Aspilia africana (Pers.) C. D. Adams (Asteraceae)2016 •
Background: Incidences of serious failures in the treatment of infectious disease by antibiotics caused by the emergence and spread of drug resistant strains of the microorganisms/multiple drug resistant bacteria have led to new global search for more effective anti-infective microbial agents from natural sources. This study intends to examine the anti-microbial potentials of the leaves of Aspilia africana, which is employed in the treatment of wounds and sores by traditional medical practitioners in Nigeria. Objective : To evaluate the anti-microbial potentials of the isolates from leaves of Aspilia africana (Pers.) C. D. Adams (Aristeraceae), using isolated clinical strains of pathogens such as Staphylococcus aureus, Methicillin Resistant Staphylococcus aureus (MRSA), Streptococcus pyogenes, Bacillus substilis, Proteus vulgaris, Salmonella typhi, Shigella dysenteriae, Escherichia coli, Klebsiella pneumonia, Candida albicans and Candida stellafoidea . Methodology: Three compounds i...
It has been estimated that over 80% of African population uses plant (herbs) regimen for treatment and control of disease due to it safety, availability, and effectiveness. In the present study, the phytochemical and antimicrobial property of Aspilia africana was investigated using standard methods. The result revealed that the plant contained appreciable amount of phytochemical which included Alkaloids (8.350±0.44), Tannins (0.618±0.06), Flavonoids (2.016±0.21), Saponin (3.218±0.55) and Phenol (0.201±0.02). The antimicrobial analysis showed that the plant had a broad spectrum effect on the test organism showing varying zones of inhibitions ranging from 9.3 to 18.4 and 7.1 to 10.4 for the ethanol and aqueous extract on bacterial isolates and 6.3 to12.3 and 7.2 to 7.8 respectively on the fungal isolate. The findings in the present study therefore suggest the use of the plant as an alternative antimicrobial agent due to its pharmacological properties.
Abtract: Problem statement: The degree of toxicity of crude extracts of Aspilia Africana (Bush marigold) leaf on the basis of routes and dosages of administration were investigated. 204 healthy, male Swiss albino mice (20-25g) were used in three consecutive studies. Approach: In the first and second study, oral and intraperitoneal doses of 100, 500, 1000, 2000 and 4000 mg kg−1 body weight of aqueous, chloroform or ethanolic extract were administered to mice. 0.2 mL of distilled water was given to the control group as placebo. Mortality and behavioural changes were monitored at 1, 2, 4, 6, 24, 48 and 96 h post administrations. In the third study, higher doses of 4,000, 8,000, 12,000, 16,000 and 20,000 mg kg−1 body weight aqueous or ethanolic extract were orally administered to fresh groups of mice. Results: Results revealed that the degree of toxicity of Aspilia africana leaf was extractant, dose and route of exposure responsive. Signs of behavioral toxicity; nervous and respiratory disorders and piloerections fluctuated in mice. Results indicated that the medium Lethal Dose (LD50) was greatest for the aqueous extract and least for the chloroform extract. Oral exposure had significantly greater LD50 (p<0.001, Av. value = 8,194.84 mg kg−1) compared to intraperitoneal exposure with an average of 232.55 mg kg−1. Conclusion: These findings support the common practice of oral administration of either aqueous or ethanolic extract of Aspilia africana as a medicinal decoction in herbal medicine. The study concludes that oral administration of up to 10,000 mg kg−1 body weight of aqueous and ethanolic extracts of Aspilia africana leaf are safe for human and animal use.
Plants from Asteraceae family are widely used for their therapeutic effects in the treatment of gastrointestinal diseases, but the consequences of excessive intake still need to be studied. The aims of this study were the evaluation of cytotoxicity, measurement of antioxidant properties and determination of polyphenolic profile of Tanacetum vulgare L. (tansy), Achillea millefolium L. (yarrow) and Solidago gigantea Ait. (goldenrod) ethanolic extracts. The cytotoxicity of extracts was monitored by xCELLigence system in real time by using porcine intestinal epithelial cell line (IPEC-1) and by measurement of changes in metabolic activity ((3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) assay). The antioxidant properties were measured by spectrophotometric methods and polyphenolic profiles were determined by HPLC-DAD for 50% ethanol extracts (10% w/v). Strong cytotoxic effect was recorded for tansy and yarrow extracts (125–1000 µg/mL) by ...
1993 •
Revista de Estudos Jurídicos UNESP
Compartilhamento De Dados e Persecução Do Crime De Branqueamento De Capitais No Âmbito Dos Paraísos Financeiros2019 •
African Journal of Infectious Diseases
Evaluation of Oraquick® HIV-1/2 as oral rapid test2013 •
Lecture Notes in Electrical Engineering
Non-dominated Sorting Manta Ray Foraging Algorithm with an Application to Optimize PD Control2021 •
Zeitschrift für romanische Philologie (ZrP)
«L’Enfant sage à trois ans» — Vom mittelalterlichen Dialog zum Volksbuch1990 •
Journal of the American College of Cardiology
An Isolated Urinary Sodium/Potassium Ratio is a Better Predictor of Progression of Hypertension Than the Estimated Daily Intake of Salt in the Chilean Population: The Association is Higher in Women2019 •
Optical and Quantum Electronics
Exploring focusing aspects of multi-regioned square zone plate2016 •
JAAD Case Reports
Pemphigus foliaceus presenting with violaceous, painful nodules and an aberrant auto-antibody expression in a Latino patient2021 •
2015 •
2008 •
Electrochimica Acta
Lignin modified glassy carbon electrode for the electrochemical determination of histamine in human urine and wine samples2014 •
Environmental Health Perspectives
Asthma among WTC Children: Registry Yields First Child Health Report2008 •
Ciência, Cuidado e Saúde
Utilização da babosa (Aloe spp) no cotidiano de usuários portadores de câncer2013 •
European Journal of Cancer Care
Surgical vocal rehabilitation after total laryngectomy2011 •