Abstract
The present study was to evaluate the antioxidant and antibacterial activity of n-hexadecanoic acid from the leaves of Ipomoea eriocarpa through aqueous ethanol extraction and further purification by column chromatography. The structure characterization of the isolated compound was elucidated by FTIR, ESI–MS, 1H-NMR, and 13C-NMR spectroscopy. The antioxidant activities of n-hexadecanoic acid were as follows: DPPH (30.19–89.13% at 100–500 µg/ml), ABTS (42.18–83.86% at 100–500 µg/ml), reducing power (0.02–0.16% at 100–500 µg/ml), nitric oxide (18.65–73.17% at 100–500 µg/ml), and superoxide (17.18–81.21% at 100–500 µg/ml) respectively. The n-hexadecanoic acid showed that moderate antibacterial activity against S. aureus, B. subtilis, E. coli, and K. pneumoniae was 7.96 \(\pm\) 0.05 mm, 10.96 \(\pm\) 0.15 mm, 11.10 \(\pm\) 0.17 mm, and 11.93 \(\pm\) 0.11 mm at the maximum concentration of 50 µg/ml. Hence, the good biological activity of the n-hexadecanoic acid from I. eriocarpa could be used as a natural antioxidant and antibacterial agent in the future pharmaceutical industry.
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The author (T.G) is grateful to Periyar University, India, for providing a university research fellowship.
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Theivanai Ganesan: extraction, purification and biological activity evaluation in whole manuscript; Murugesan Subban: supervised the work and guidance; Denzil Britto Christopher Leslee: NMR analysis; Shanmuga Bharathi Kuppannan: NMR interpretation; Palaniappan Seedevi: biological and chemical analysis.
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Ganesan, T., Subban, M., Christopher Leslee, D.B. et al. Structural characterization of n-hexadecanoic acid from the leaves of Ipomoea eriocarpa and its antioxidant and antibacterial activities. Biomass Conv. Bioref. (2022). https://doi.org/10.1007/s13399-022-03576-w
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DOI: https://doi.org/10.1007/s13399-022-03576-w