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Isolation of Potential Compound from the Leaves of Elytraria acaulis and Evaluating Its Therapeutic Properties Using In Vitro Studies Against Ovarian Cancer

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Abstract

The present study was designed to isolate a potential compound from the extracts of Elytraria acaulis (E. acaulis) for ovarian cancer. n-Hexane, ethyl acetate, chloroform, acetone and methanol extract were taken using the Soxhlet method. Thin layer, column chromatography, NMR and MASS studies were done for the isolation and structural characterization of the compound. Finally, the novel compound (Z)-3-(2-methyl-3-oxoprop-1-en-1-yl) phenyl heptanoate was identified. MTT assay, cell morphology and cell cycle analysis were done to evaluate the anticancer property of the compound. In the MTT assay, the percentage of the cell viability treated with the isolated compound was decreased while increasing the concentration of the compound. Cancer cells treated with the isolated compound showed distinct morphological changes when compared to the control untreated cells. In the cell cycle analysis, the isolated compound induced a significant increase in the percentage of cells in G0/G1 phase and a decrease in the percentage of cells in the S phase and G2-M phase of the PA 1 cell lines. The cell cycle arrest induced by the isolated compound may account for its antiproliferative capacity. Hence, the novel compound isolated from E. acaulis can be a potent candidate in the designing of anticancer drugs.

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All data available from corresponding author by request.

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Authors and Affiliations

  1. PG & Research Department of Zoology, Pachaiyappa’s College, Chennai, 600 030, Tamil Nadu, India

    P. Priya

  2. PG & Research Department of Chemistry, Pachaiyappa’s College, Chennai, 600 030, Tamil Nadu, India

    M. Anbarasu & V. Sivamurugan

  3. Department of Biotechnology, Faculty of Biomedical Sciences and Technology, SRI RAMACHANDRA Institute of Higher Education and Research (SRIHER), Deemed to Be University (DU), Chennai, 600 116, Tamil Nadu, India

    V. S. Christina

  4. PG and Research Department of Zoology, C. Abdul Hakeem College (Autonomous), Melvisharam, 632 509, Tamil Nadu, India

    S. Abdul Majeed

  5. Central Research Facility (CRF), SRI RAMACHANDRA Institute of Higher Education and Research (SRIHER), Deemed to Be University (DU), Chennai, 600 116, Tamil Nadu, India

    R. Lakshmi Sundaram

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Contributions

PP: conceptualisation, manuscript writing, formal analysis; MA: compound isolation; VSC: anticancer studies; SAM: formal analysis; VS: spectral analysis and writing; RLS: characterisation.

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Correspondence to P. Priya or V. Sivamurugan.

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Priya, P., Anbarasu, M., Christina, V.S. et al. Isolation of Potential Compound from the Leaves of Elytraria acaulis and Evaluating Its Therapeutic Properties Using In Vitro Studies Against Ovarian Cancer. Appl Biochem Biotechnol 194, 5607–5626 (2022). https://doi.org/10.1007/s12010-022-04048-7

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