Antibacterial Activities of Monsonia Angustifolia and Momordica Balsamina Linn Extracts against Carbapenem-Resistant Acinetobacter Baumannii
Abstract
:1. Introduction
2. Results
3. Materials and Methods
3.1. Ethical Approval
3.2. Bacteria Collection, Identification and Antimicrobial Susceptibility Testing
3.3. Molecular Characterization of Isolate and Identification
3.4. Collection of Plant Material
3.5. Plant Preparation and Storage
3.5.1. Momordica Balsamina Linn
3.5.2. Monsonia Angustofolia
3.6. Preparation of Extracts
3.6.1. Momordica Balsamina Linn
3.6.2. Monsonia Angustofolia
3.7. Screening for Antibacterial Activity
3.7.1. Disc Diffusion
3.7.2. Thin Layer Chromatography Coupled with Bioautography and Retention Factor Calculation
3.8. Determination of Minimum Inhibitory Concentration (MIC)
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Plants | Testing Method | Solvents | |||
---|---|---|---|---|---|
Hexane | DCM | Acetone | Methanol | ||
Momordica balsamina L | K.B.D.D. TLC/Bioautography | - - | - - | - - | + - |
Monsonia angustofolia | K.B.D.D. TLC/Bioautography | - + | - + | - + | - + |
Scheme | Hexane | Dichloromethane | Acetone | Methanol |
---|---|---|---|---|
Rf Value | 0.05 0.17 0.39 - - - - - - | 0.58 0.78 0.36 0.48 0.5 0.56 0.67 0.78 0.9 | 0.11 0.56 0.24 0.37 - - - - - | 0.11 0.27 - - - - - - - |
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Nogbou, N.-D.; Mabela, D.R.; Matseke, B.; Mapfumari, N.S.; Mothibe, M.E.; Obi, L.C.; Musyoki, A.M. Antibacterial Activities of Monsonia Angustifolia and Momordica Balsamina Linn Extracts against Carbapenem-Resistant Acinetobacter Baumannii. Plants 2022, 11, 2374. https://doi.org/10.3390/plants11182374
Nogbou N-D, Mabela DR, Matseke B, Mapfumari NS, Mothibe ME, Obi LC, Musyoki AM. Antibacterial Activities of Monsonia Angustifolia and Momordica Balsamina Linn Extracts against Carbapenem-Resistant Acinetobacter Baumannii. Plants. 2022; 11(18):2374. https://doi.org/10.3390/plants11182374
Chicago/Turabian StyleNogbou, Noel-David, Dimpho Raesibe Mabela, Buang Matseke, Ntwanano Sipho Mapfumari, Mmammosheledi Elsie Mothibe, Lawrence Chikwelu Obi, and Andrew Munyalo Musyoki. 2022. "Antibacterial Activities of Monsonia Angustifolia and Momordica Balsamina Linn Extracts against Carbapenem-Resistant Acinetobacter Baumannii" Plants 11, no. 18: 2374. https://doi.org/10.3390/plants11182374