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2016, Herbal Medicine: Open Access
2007 •
We estimate the genome size of V. vinifera to be 504.6 Mb. Genomic sequences corresponding to 477.1 Mb were assembled in 2,093 metacontigs and 435.1 Mb were anchored to the 19 linkage groups (LGs). The number of predicted genes is 29,585, of which 96.1% were assigned to ...
The widespread adoption of NGS technologies has created in recent years an unparalleled inflow of sequence data, which can be leveraged to obtain clear information on the genetics and transcriptome of various organisms. In agronomics, these new methods can be used to gather information on cultivars of sequenced organisms. In particular we were interested in identifying putative specific genes represented in Vitis vinifera cv Corvina and not in the sequence grape clone (Pinot Noir 40024). These genes could be of interest in order to investigate the molecular bases of specific quality characteristics. To this end, we have pooled RNA of Vitis vinifera cv. Corvina samples taken from 45 different tissues and/or development stages, and sequenced it with an Illumina HiSeq platform. We obtained more than 100 million reads, which were filtered and assembled with the Velvet/Oases software suite. To facilitate the assembly, we provided the software with the alignment of the reads against the V...
Until recently, there was no data available on the genome sequences of medicinal plants. But now, public databases for the transcriptomes of important medicinal plants are available. But an analysis pipeline effectively combining publicly available tools is lacking which would otherwise enable in-depth analysis of the transcriptomes. In this context, we have developed an effective in silico analysis pipeline using tools such as FastQC, FastQ Groomer, TopHat, Cufflinks, Cuffmerge and Cuffdiff available in Galaxy platform and other tools such as DAVID, ExPASy, MetaCyc and PlantCyc. We have tested the pipeline for comparative analysis of the transcriptome of Catharanthus roseus (L.) G.Don and Rauvolfia serpentina (L.) Benth. ex Kurz, two well-known medicinal plants. This study identified genes that are similarly expressing in the roots of these plants leading to the formation of the same secondary metabolite, " Strictosidine " and also identified differentially expressing genes in the leaves of C. roseus and R. serpentina lead to the formation of different metabolites, " Vinblastine " and " Ajmaline ". The findings of the study indicated that the pipeline developed is effective and helped to analyze the transcriptomes and expression data.
Scientific Reports
The first draft genome of Picrorhiza kurrooa, an endangered medicinal herb from Himalayas2021 •
Picrorhiza kurrooa is an endangered medicinal herb which is distributed across the Himalayan region at an altitude between 3000–5000 m above mean sea level. The medicinal properties of P. kurrooa are attributed to monoterpenoid picrosides present in leaf, rhizome and root of the plant. However, no genomic information is currently available for P. kurrooa, which limits our understanding about its molecular systems and associated responses. The present study brings the first assembled draft genome of P. kurrooa by using 227 Gb of raw data generated by Illumina and PacBio RS II sequencing platforms. The assembled genome has a size of n = ~ 1.7 Gb with 12,924 scaffolds. Four pronged assembly quality validations studies, including experimentally reported ESTs mapping and directed sequencing of the assembled contigs, confirmed high reliability of the assembly. About 76% of the genome is covered by complex repeats alone. Annotation revealed 24,798 protein coding and 9789 non-coding genes. ...
Journal of Shahrekord University of Medical Sciences
The prevalence sarcocystis infection in slaughtered animals in slaughterhouse of Shahrekord using histhopathological method 20082010 •
2014 •
En este trabajo se evaluo la absorcion de metales biodisponibles mediante el uso de hidrogeles de poliacrilamida sumergidos en aguas intersticiales del Golfo de Cariaco. Los hidrogeles se sintetizaron mediante polimerizacion radical de acrilamida (AAm), usando como agente entrecruzante N,N�metilenbisacrilamida y como iniciador persulfato de amonio. En el hidrogel se midio la capacidad de absorcion mediante su indice de hinchamiento que fue de 649% en agua de mar artificial. Se comprobo mediante microscopia electronica de barrido (MEB), que tal comportamiento fue generado por su morfologia de tipo �esponja�, caracteristica de una estructura superabsorbente. Se analizo la capacidad de los polimeros de interaccionar con los iones presentes en las aguas de los sedimentos superficiales recolectadas en siete estaciones. Las concentraciones de los metales totales se cuantificaron mediante espectrometria de emision optica acoplado inductivamente a un plasma (ICPOES), al igual que los metale...
O presente trabalho busca investigar os meios de projecao de poder no espaco cibernetico e como e explorado; assim como o seu impacto na politica do sistema internacional. Essa pesquisa ira analisar os impactos do escândalo de espionagem de 2013 na politica publica brasileira daquela epoca. Assim como considerar as formas de conexao e informacao, legitimas ou nao. No desenvolvimento do trabalho serao analisados impactos das politicas publicas brasileiras que tratam da defesa cibernetica sobre as atividades de espionagem cibernetica do seculo XXI, por meio de uma revisao historica da evolucao da espionagem da Guerra Fria ate os dias atuais e analises dos impactos do espaco cibernetico sobre as atividades de espionagem. Assim como entender as distincoes das atividades de inteligencia e espionagem, com enfase em meios informais de coleta de informacao, como as distâncias e embaixadas. Identificar, nas politicas publicas brasileiras para a seguranca e defesa ciberneticas, pontos de miti...
Experimental Biology and Medicine
A BRIEF COMMUNICATION: Upregulation of Interferon-Induced Genes in Infants with Virus-Associated Acute Bronchiolitis2007 •
To determine whether there is an airway IFN response in infants with acute bronchiolitis and to establish whether the rate of such a response is related to the severity of illness, the expression of some IFN-induced genes was measured in nasopharyngeal washes from 39 infants with acute bronchiolitis. The results indicate that in infants with a virus-associated acute bronchiolitis there is a strong activation of IFN system and that the severity of illness is inversely related to the level of expression of IFN-induced genes. This suggests that the IFN response plays an important role in determining virus–associated respiratory disease in early life.
Journal of General Virology
Genotype C of hepatitis B virus can be classified into at least two subgroups2004 •
A genomic characterization of hepatitis B virus (HBV) was done for 56 pre-S1/pre-S2 genes and 10 full-length HBV genotype C isolates from five Asian countries. Phylogenetic analysis of the pre-S1/pre-S2 genes revealed two major groups within genotype C: one for isolates from southeast Asia including Vietnam, Myanmar and Thailand (named HBV/C1) and the other for isolates from Far East Asia including Japan, Korea and China (named HBV/C2). This finding was confirmed by phylogenetic analysis based on the full-length sequence of 32 HBV genotype C isolates, including 22 from database entries. Two isolates from Okinawa, the island off the southern end of Japan, formed a different branch. Specific amino acid sequence changes were identified in the large S protein (amino acids 51, 54, 60, 62 and 73) and P protein (amino acids 231, 233, 236, 248, 252 and 304). Our results indicate that genotype C of HBV can be classified into at least two subgroups.
Conceptual Modeling
Domain Ontology As Conceptual Model for Big Data Management: Application in Biomedical Informatics2014 •
IEEE Transactions on Wireless Communications
Optimization of cooperative spectrum sensing with energy detection in cognitive radio networks2009 •
Clinical Nutrition
Protein synthesis after experimental injury in pigs. A comparison of infusion solutions with different amino acid patterns1982 •
Journal of Industrial and Engineering Chemistry
Production of diacylglycerols by esterification of oleic acid with glycerol catalyzed by diatomite loaded SO 4 2− /TiO 22017 •
2006 •
2018 •
The American journal of case reports
Double Trouble - Severe Hypernatremia Secondary to Central Diabetes Insipidus Complicated by Hypercalcemic Nephrogenic Diabetes Insipidus: A Case Report2018 •
LATAM Revista Latinoamericana de Ciencias Sociales y Humanidades
Estilos de pensamiento y enfoques de enseñanza en docentes de educación primaria de Cuenca, Ecuador2014 •
Revista de investigación histórica REGISTROS - Universidad Nacional de Mar del Plata (Mar del Plata, Argentina)
Reseña : "Claude Parent: Les desseins d’un architecte" AUDREY JEANROY, 20222023 •
Journal of Physical Oceanography
A Global Ocean-Atmosphere Climate Model. Part II. The Oceanic Circulation1975 •
Ensiklopedia Research and Community Service Review
Tinjauan Efektivitas Penerapan Simpang Bersinyal DI Simpang Bypass Manggis Kota Bukittinggi2012 •