Abstract
Horsegram (Macrotyloma uniflorum) is a legume species widely distributed in Indian subcontinent and an important supplement of dietary protein with medicinal effects. The genus Macrotyloma consists of 25 species, and most of them are wild except Macrotyloma uniflorum spp. uniflorum, which is cultivated in many parts of India. Horsegram is used as an important pulse crop since seeds are rich in protein and consumed in majority by poorest section of the society. Horsegram seeds are an excellent source of protein, carbohydrates, essential amino acids, energy, iron, molybdenum, phosphorus, iron and vitamin C. Its seeds are reported to be high in tannins and polyphenols as compared to other legumes and therefore considered as a good source of antioxidant. Despite nutritional advantages over other legumes, the crop is not grown commercially due to the presence of many undesirable traits in cultivated horsegram such as twining and indeterminate growth habit, long and thin stem and very late and non-synchronous maturity. Probable progenitor of cultivated horsegram is considered as M. axillare, which possesses many desirable traits such as higher number of seeds per plant and seed yield per plant and resistance against many pathogens, but due to non-compatibility with cultivated horsegram the traits cannot be transferred using conventional breeding strategies. The genome structure and organization of this crop are poorly understood, thereby limiting the effective use of gene resources for genetic improvement. The development of genomic resources in Macrotyloma uniflorum was started with the study on transferability of genomic SSR and COS markers from the related well-characterized legume species such as Medicago truncatula, Trifolium pratense and Cajanus cajan. In addition, the available sequence information in NCBI database was downloaded and used to design 63 SSR and 27 IPL markers. The first systematic attempt to develop genomic resources in horsegram was made by mining of transcriptome sequence data, and 3342 SSRs were developed and validated on the diverse panel of horsegram lines. Next-generation sequencing platform was used to develop draft genome sequence in this crop and reported the size of genome is 398 Mb. The whole genome sequence data was also used to develop genomic resources in horsegram, and 5755 genomic SSRs were designed and validated. All these genomic resources are being utilized for the diversity and structure analysis of germplasm, construction of linkage map and identification of genomic regions associated with different traits. The first framework linkage map of Macrotyloma uniflorum was constructed using 211bmolecular markers on 190 RILs progeny derived from a narrow cross between lines HPK4 and HPKM249. The linkage map showed ten linkage groups that spanned 1423.4cM with a mean marker interval of 9.6cM. Five QTLs related to drought-related parameters and seed yield were detected on five LGs with an LOD threshold of 4.0. The linkage and QTL analysis being carried in this crop can be useful for future research work pertaining to construction of highly enriched genetic maps as well as development of drought-resistant and high-yielding varieties in horsegram using marker-assisted selection.
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References
Aditya JP, Bhartiya A, Chahota RK, Joshi D, Chandra N, Kant L, Pattanayak A (2019) Ancient orphan legume horse gram: a potential food and forage crop of future. Planta 250:891–909. https://doi.org/10.1007/s00425-019-03184-5
Agarwal M, Shrivastava N, Padh H (2008) Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Rep 27:617–631
Allen ON, Allen EK (1981) The Leguminosae. A source book of characteristics, uses and nodulation. The University of Wisconsin Press, Madison
Anderson JW, Smith BM, Gustafson NJ (1994) Health benefits and practical aspects of high-fiber diets. Am J Clin Nutr 59:1242–1247
Asha KI, Latha M, Abraham Z, Jayan PK, Nair MC, Mishra SK (2006) Genetic resources. In: Kumar D (ed) Horsegram in India. Scientific Publisher, Jodhpur, pp 11–28
Babayeva S, Akparov Z, Abbasov M, Mammadov A, Zaifizadeh M, Street K (2009) Diversity analysis of Central Asia and Caucasian lentil (Lens culinaris Medik.) germplasm using SSR fingerprinting. Genet Resour Crop Evol 56:293–298
Balasubramanian V (1985) Horsegram. Central Research Institute for Dry land Agriculture, Hyderabad, pp 183–197
Bhadana VP, Sharma PK, Ansari MA, Baishya LK, Punetha P, Datt S, Prakash N, Rana KS (2013) Food legumes for livelihood and nutritional security in North Eastern Himalayan region: prospects and constraints. Indian J Agric Sci 83(9):899–906
Bhardwaj J, Chauhan R, Swarnkar MK, Chahota RK, Singh AK, Shankar R, Yadav SK (2013) Comprehensive transcriptomic study on horsegram (Macrotyloma uniflorum): De novo assembly, functional characterization and comparative analysis in relation to drought stress. BMC Genomics 14:647–664
Bhartiya A, Aditya JP, Kant L (2015) Nutritional and remedial potential of an underutilized food legume horse gram (Macrotyloma uniflorum): a review. J Anim Plant Sci 25(4):908–920
Bhatt R, Karim AA (2009) Exploring the nutritional potential of wild and underutilized legumes. Compr Rev Food Sci Food Saf 8:305–331
Blair MW, Torres MM, Martha C, Giraldo MC, Pedraza F (2009) Development and diversity of Andean-derived, gene-based microsatellites for common bean (Phaseolus vulgaris L.). BMC Plant Biol 9:100
Blair MW, Hurtado N, Chavarro CM, Torres MCM, Giraldo MC, Pedraza F, Tomkins J et al (2011) Wing R Gene-based SSR markers for common bean (Phaseolus vulgaris L.) derived from root and leaf tissue ESTs: an integration of the BMC series Blair. BMC Plant Biol 11:50–51
Blumenthal MJ, Staples IB (1993) Origin, evaluation and use of Macrotyloma as forage – a review. Trop Grassl 27:16–29
Bolbhat SN, Dhumal KN (2012) Physiological, biochemical and enzymological studies in horsegram (Macrotyloma uniflorum (Lam.) Verdc.). Int J Adv Sci Tech 6(2):679–689
Bravo L, Siddhuraju P, Saura-Calixto F (1999) Composition of underexploited Indian pulses. Comparison with common legumes. Food Chem 64:185–192
Brink M (2006) Macrotyloma uniflorum (Lam.) Verdc. In: Brink M, Belay G (eds) PROTA 1: cereals and pulses PROTA, Wageningen
Burle ML, Fonseca JR, Kami JA, Gepts P (2010) Microsatellite diversity and genetic structure among common bean (Phaseolus vulgaris L.) landraces in Brazil, a secondary center of diversity. Theor Appl Genet 121:801–813
Chahota, RK (2009) Induction of early flowering and other important traits in horse gram (Macrotyloma uniflorum) using gamma irradiation. Final project report submitted to Bhabha Atomic Research Centre, Trombay, India
Chahota RK, Sharma TR, Dhiman KC, Kishore N (2005) Characterization and evaluation of horsegram (Macrotyloma uniflorum Roxb.) germplasm from Himachal Pradesh. Indian J Plant Genet Resour 18(2):221–223
Chahota RK, Sharma TR, Sharma SK, Kumar N, Rana JC (2013) Horsegram. In: Singh M, Upadhyaya HD, Bisht IS (eds) Genetic and genomic resources of grain legume improvement. Elsevier, 32 Jamestown Road London, 293–305
Chahota RK, Shikha D, Rana M, Sharma V, Nag A, Sharma TR, Rana JC, Hirakawa H, Isobe S (2017) Development and characterization of SSR markers to study genetic diversity and population structure of horsegram germplasm (Macrotyloma uniflorum). Plant Mol Biol Report 35:550–561
Chahota RK, Sharma V, Rana M, Sharma R, Choudhary S, Sharma TR, Shirasawa K, Hirakawa H, Sachiko N. Isobe, (2020) Construction of a framework linkage map and genetic dissection of drought- and yield-related QTLs in horsegram (Macrotyloma uniflorum). Euphytica 216 (4)
Choudhary S, Gaur R, Gupta S, Bhatia S (2012) EST-derived genic molecular markers: development and utilization for generating an advanced transcript map of chickpea. Theor Appl Genet 124:1449–1462
Cook BG, Pengelly BC, Brown SD, Donnelly JL, Eagles DA, Franco MA, Hanson J, Mullen BF, Partridge IJ, Peters M, Schultze- Kraft R (2005) Tropical Forages: an interactive selection tool.[CD-ROM], CSIRO, DPI&F (QLD), CIAT and ILRI, Brisbane, Australia
Cullis C, Kunert KJ (2017) Unlocking the potential of orphan legumes. J Exp Bot 68(8):1895–1903
Dai X, Xu Y, Ma Q, Xu W, Wang T, Xue Y, Chong K (2007) Overexpression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis. Plant Physiol 143:1739–1751
Deodhar SY (2016) Orphan food? Nay, future of food understanding the pulse of the indian market. Indian Institute of Management, Ahmedabad, India. https://web.iima.ac.in/assets/snippets/workingpaperpdf/15644916822016–09-01.pdf. Accessed 27 Mar 2019
Doyle JJ, Luckow MA (2003) The rest of the iceberg. Legume diversity and evolution in a phylogenetic context. Plant Physiol 131:900–910
Dutta S, Kumawat G, Singh BP, Gupta DK, Singh S, Dogra V, Gaikwad K, Sharma TR, Raje RS, Bandhopadhya TK, Datta S, Singh MN, Bashasab F, Kulwal P, Wanjari KB, Varshney RK, Cook DR, Singh NK (2011) Development of genic-SSR markers by deep transcriptome sequencing in pigeon pea [Cajanus cajan (L.) Millspaugh]. BMC Plant Biol 11:17
Ellis N (2016) Breeding and genetics for improved productivity and resilience. 10-year research strategy for pulse crops. International year of pulses. http://grainlegumes.cgiar.org/wp-content/uploads/2017/01/IYP
Foyer CH, Lam HM, Nguyen HT et al (2016) Neglecting legumes has compromised global food and nutritional security. Nat Plants 2:16112
Fuller DQ, Murphy C (2018) The origins and early dispersal of horsegram (Macrotyloma uniflorum), a major crop of ancient India. Genet Resour Crop Evol 65:285–305
Gahlan P, Singh HR, Shankar R, Sharma N, Kumari A, Chawla V, Ahuja PS, Kumar S (2012) De novo sequencing and characterization of Picrorhiza kurroa transcriptome at two temperatures showed major transcriptome adjustments. BMC Genomics 13:126–110
Gaur RD, Dangwal LR (1997) A new species of macrotyloma from Garhwal Himalaya, U.P., India. J Bombay Nat Hist Soc 94:381–383
Gept P, Beavis WD, Brummer EC, Shoemaker RC, Stalker HT, Weeden NF et al (2005) Legumes as a model plant family. Genomics for food and feed report of the cross legumes advances through genomics conference. Plant Physiol 137:1228–1235
Ghorpade VM, Kadam SS, Salunkhe DK (1986) Thermal stability and changes in trypsin inhibitor during germination and cooking of horse gram. J Food Sci Technol 23:164–165
Govind G, Harshavardhan VT, ThammeGowda HV, Patricia JK, Kalaiarasi PJ, Dhanalakshmi R, Iyer DR, Senthil KM, Muthappa SK, Sreenivasulu N, Nese S, Udayakumar M, Makarla UK (2009) Identification and functional validation of a unique set of drought induced genes preferentially expressed in response to gradual water stress in peanut. Mol Gen Genomics 281:591–605
Guimaraes PM, Brasileiro ACM, Morgante CV, Martins ACQ, Pappas G, Silva OB, Togawa R, Leal-Bertioli SCM, Araujo ACG, Moretzsohn MC, Bertioli DJ (2012) Global transcriptome analysis of two wild relatives of peanut under drought and fungi infection. BMC Genomics 13:387–310
Gupta VP, Sharma SK, Rathore PK (1994) Induced mutagenic response of gamma irradiation on horsegram (Macrotyloma uniflorum). Indian J Agric Sci 64:160–164
Gupta SK, Bansal R, Gopalakrishna T (2012) Development of intron length polymorphism markers in cowpea [Vigna unguiculata (L.) Walp.] and their transferability to other Vigna species. Mol Breed 30(3):1363–1370
Haq N (2002) In: Haq N (ed) Fruits for the future in Asia. Proceedings of a regional consultation meeting on utilization of tropical fruit trees in Asia, Bangkok, Thailand. ICUC activities in South Asia. 16 p
Hirakawa H, Chahota RK, Shirasawa K, Sharma TR, Isobe S (2017, May 29–31) Draft genome sequencing of horsegram (Macrotyloma uniflorum); PAG Asia, South Korea
Hiremath PJ, Farmer A, Cannon SB, Woodward J, Kudapa H, Tuteja R, Kumar A, Bhanuprakash A, Mulaosmanovic B, Gujaria N, Krishnamurthy L, Gaur PM, Kavikishor PB, Shah T, Srinivasan R, Lohse M, Xiao Y, Town CD, Cook DR, May GD, Varshney RK (2011) Large-scale transcriptome analysis in chickpea (Cicer arietinum L.), an orphan legume crop of the semi-arid tropics of Asia and Africa. Plant Biotechnol 9:922–931
IPGRI (2002) Neglected and underutilized plant species: strategic action plan of the International Plant Genetic Resources Institute. International Plant Genetic Resources Institute, Rome
Jamadagani BM, Birari SP (1996) Modification in photoperiodic response of flowering by gamma-rays induced mutation in horsegram. J Maharashtra Agric Univ 21:384–386
Kadam SS, Salunkhe DK (1985) Nutritional composition, processing, and utilization of horse gram and moth bean. Crit Rev Food Sci Nutr 22(1):1–26
Kaldate R, Rana M, Sharma V, Hirakawa H, Kumar R, Singh G, Chahota RK, Isobe SN, Sharma TR (2017) Development of genome-wide SSR markers in horsegram and their use for genetic diversity and cross-transferability analysis. Mol Breed 37:103
Kamei CLA, Severing EI, Dechesne EI, Furrer H, Dolstra O, Trindade LM (2016) Orphan crops browser: a bridge between model and orphan crops. Mol Breed 36:9. https://doi.org/10.1007/s11032-015-0430-2
Kang MJ, Kim JI, Yoon SY, Kim JC, Cha IJ (2006) Pinitol from soybeans reduces postprandial blood glucose in patients with type 2 diabetes mellitus. J Med Food 9:182–186
Katiyar RP (1984) Kulthi a promising pulse crop for Himachal Hills. Indian Farm 34(9):31–35
Kawale SB, Kadam SS, Chavan UD, Chavan JK (2005) Effect of processing on insoluble dietary fiber and resistant starch in kidney bean and horsegram. J Food Sci Technol 42:361–362
Kulkarni GB (2010) Evaluation of genetic diversity of horse gram (Macrotyloma uniflorum) germplasm through phenotypic trait analysis. Green Farm 1(6):563–565
Kulkarni GB, Mogle UP (2011) Characterization and evaluation of horse gram (Macrotyloma uniflorum (Lam.) Verdcourt) genotypes. Sci Res Rep 1(3):122–125
Kumar D (2005) Status and direction of arid legumes research in India. Indian J Agric Sci 75:375–391
Lackey JA (1981) Phaseolus. In: Polhill RM, Raven PH (eds) Advances in legume
Liu J, Guan JP, Xu DX, Zhang XY, Gu J, Zong XX (2008) Genetic diversity and population structure in lentil (Lens culinaris Medik.) germplasm detected by SSR markers. Acta Agron Sin 34:1901–1909
Magbagbeola JAO, Adetoso JA, Owolabi OA (2010) Neglected and underutilized species (NUS): a panacea for community focused development to poverty alleviation/poverty reduction in Nigeria. J Econ Int Financ 2(10):208–211
Mahajan V, Shukla SK, Gupta NS, Majumdera D, Tiwari V, Piiasad SVS (2007) Identifying phenotypically stable genotypes and developing strategy far yield improvement in horsegram (Macrotyloma uniflorum) for mid-altitudes of Northwestern Himalayas. Indian J Agric Sci 3:19–22
Mall TP (2017) Diversity of potential orphan plants in health management and climate change mitigation from Bahraich (Uttar Pradesh). India Int J Curr Res Biosci Plant Biol 4(11):106–145
Mathew KM (1983) The flora of Tamil Nadu Carnatic. Part one Polypetalae. The Rapinat Herbarium, St. Joseph’s College, Tiruchirapalli
Morris JB (2008) Macrotyloma axillare and M. uniflorum: descriptor analysis, anthocyanin indexes, and potential uses. Genet Resour Crop Evol 55:5–8
Nair NC, Henry AN (1983) Flora of Tamil Nadu. India series I: analysis, vol 1. Botanical Survey of India Southern Circle, Coimbatore, pp 1–184
National Academy of Sciences (1978) Mothbean in tropical legumes: resources for the future. National Academy of Sciences, Washington, DC
Negi KS, Yadav S, Mandal S, Bhandari DC (2002) Registration of wild horsegram germplasm (INGR No. 02007; IC2I2722). Indian J Agric Sci 15(3):300–301
Paux E, Soudille P, Mackay I, Feuillet C (2012) Sequence-based marker development in wheat: advances and applications to breeding. Biotechnol Adv 30:1071–1088
Peakall R, Gilmore S, Keys W, Morgante M, Rafalski A (1998) Cross species amplification of soybean (Glycine max) simple sequence repeat (SSRs) within the genus and other legume genera: implication for transferability of SSRs in plants. Mol Biol Evol 15:1275–1287
Prakash BG, Hiremath CP, Devarnavdgi SB, Salimath PM (2010) Assessment of genetic diversity among germplasm lines of horsegram (Macrotyloma uniflorum) at Bijapur. Electron J Plant Breed 1:414–419
Pugalenthi M, Vadivel V, Siddhuraju P (2005) Alternative food/feed perspectives of an underutilized legume Mucuna pruriens var. utilis-a review. Plant Foods Hum Nutr 60:201–218
Pullaiah T, Chennaiah E (1997) Flora of Andhra Pradesh (India), Rananculace-Alangiaceae, vol 1. Scientific Publishers, Jodhpur
Ramakanth RS, Setharama A, Patil NM (1979) Induced mutations in dolichos lablab. Mutat Breed Newslett 14:1–2
Rana JC (2010) Evaluation of horsegram germplasm. Annual progress report of the NBPGR, Shimla, India
Reddy PCO, Sairanganayakulu G, Thippeswamy M, Reddy PS, Reddy MK, Sudhakar C (2008) Identification of stress- induced genes from the drought tolerant semi- arid legume crop horsegram [Macrotyloma uniflorum (Lam.) Verdc.] through analysis of subtracted expressed sequence tags. Plant Sci 175(3):372–384
Ribaut JM, Hoisington D (1998) Marker-assisted selection: new tools and strategies. Trends Plant Sci 3:236–239
Salini K, Maruthi V, Maheswari M, Sarkar B (2014) Genetic improvement of horse gram through mutation breeding. 3rd international conference on agriculture & horticulture, October 27–29, Hyderabad International Convention Centre, India
Sharma RK (1995) Nature of variation and association among grain yield and yield components in horsegram. J Crop Improv 22(1):73–76
Sharma V, Rana M, Katoch M, Sharma PK, Ghani M, Rana JC, Sharma TR, Chahota RK (2015a) Development of SSR and ILP markers in horsegram (Macrotyloma uniflorum), their characterization, cross-transferability and relevance for mapping. Mol Breed 35:102
Sharma V, Sharma TR, Rana JC, Chahota RK (2015b) Analysis of genetic diversity and population structure in horsegram (Macrotyloma uniflorum) using RAPD and ISSR markers. Agric Res 4:221–230
Sodani SN, Paliwal RV, Jain LK (2004) Phenotypic stability for seed yield in rainfed horsegram (Macrotyloma uniflorum (Lam.) Verdc.) Paper presented in natural symposium on arid legumes for sustainable agriculture and trade, 5–7 Nov., Jodhpur
Sreenivasan E (2003) Hybridization studies in horse gram. In: Henry A, Kumar D, Singh NB (eds) Advances in arid legumes research. Scientific Publishers, Jodhpur, pp 115–121
Staples IB (1966) A promising new legume. Queensland J Agric Anim Sci 92:388–392
Staples IB (1982) Intra and interspecific crosses in Macrotyloma axillare. Proceedings of the Second Australian Agronomy Conference, p. 340, Wagga
Subba Rao A, Sampath SR (1979) Chemical composition and nutritive value of horsegram (Dolichos biflorus). Mysore. J Agric Sci 13:198–205
Sudha N, Mushtari Begum J, Shambulingappa KG, Babu CK (1995) Nutrients and some anti-nutrients in horsegram (Macrotyloma uniflorum (Lam.) Verdc.) Food Nutr Bull 16: 81–83 systematic Part I (pp. 301–327). Kew, London: Royal Botanic Garden
Thudi Y, Li S, Jackson GD, Varshney RK (2011) Current state-of-art of sequencing technologies for plant genomics research. Brief Funct Genomics 11:3–11
Tripathi AK (1999) Variability in horsegram. Ann Agric Sci 20:382–383
Uma RK, Narayanaswamy S, Nethra N, Prasad RS (2013) Utilization of SSR markers for identification of horsegram [Macrotyloma uniflorum (Lam.)] genotypes. Ann Plant Sci 2(12):556–562
Van-Verk MCV, Bol JF, Linthorst HJM (2011) WRKY transcription factors involved in activation of SA biosynthesis genes. BMC Plant Biol 11:89–10
Varshney RK, Sigmund R, Borner A, Korzun V, Stein N, Sorrels ME, Langridge P, Graner A (2005) Interspecific transferability and comparative mapping of barley EST-SSR markers in wheat, rye and rice. Plant Sci 168:195–202
Vavilov NI (1951a) Phytogeographic basis of plant breeding. Chron Bot 13:13–54
Vavilov NI (1951b) The origin, variation, immunity and breeding of cultivated crops. Chron Bot 13:364
Verdcourt B (1971) Phaseoleae. In: Gillet JC, Polhill RM, Verdcourt V (eds) Flora of east tropical Africa. Leguminosae–subfamily Papilionoideae, vol 2. Crown Agents, pp 581–594
Wang X, Basnayake BMVS, Zhang H, Li G, Li W, Virk N, Mengiste T, Song F (2009) The Arabidopsis ATAF1, a NAC transcription factor, is a negative regulator of defense responses against necrotrophic fungal and bacterial pathogens. Mol Plant Microbe Interact 22:1227–1238
Wang Z, Libault M, Joshi T, Valliyodan B, Nguyen HT, Xu D, Stacey G (2010) Soy DB: a knowledge database of soybean transcription factors. BMC Plant Biol 10:14–10
Xu SC, Gong YM, Mao WH, Hu QZ, Zhang GW, Fu W, Xian QQ (2012) Development and characterization of 41 novel EST-SSR markers for Pisum sativum (Leguminosae). Am J Bot 99(4):e149–e153
Yadav S, Negi KS, Mandal S (2004) Protein and oil rich wild horsegram. Genet Resour Crop Evol 51(6):629–633
Zeven AC, DeWet JMJ (1982) Dictionary of cultivated plants and their regions of diversity. Center for Agricultural Publishing and Documentation, Wageningen
Zohary D (1970) Centers of diversity and centers of origin. In: Frankel OH, Bennett F (eds) Genetic resources in plants. Blackwell Scientific Publications, Oxford, pp 33–42
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Chahota, R.K., Thakur, N., Sharma, R. (2020). Efficient Improvement in an Orphan Legume: Horsegram, Macrotyloma uniflorum (Lam.) Verdi, Using Conventional and Molecular Approaches. In: Gosal, S.S., Wani, S.H. (eds) Accelerated Plant Breeding, Volume 3. Springer, Cham. https://doi.org/10.1007/978-3-030-47306-8_12
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