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Conductimetric Analysis and Preliminary Evaluation of Phytochemical Constituents of Sporobolus spicatus (Vahl) Kunth [Family: Poaceae] by FTIR and UV–Vis Spectroscopic Techniques

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Chemistry for a Clean and Healthy Planet (ICPAC 2018)

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Abstract

FTIR and UV–Vis techniques, being essential tools for analytical and research applications, were used to provide first-hand spectroscopic information on the phytochemical constituents present in Sporobolus spicatus, a halophytic plant commonly referred to as salt grass. The plant sample used in the analysis was collected from the Okavango Delta area, Republic of Botswana. Preliminary phytochemical screening on the whole plant parts shows positive tests for flavonoid and phenolic compounds but absence of saponins. FTIR method in the mid-infrared region 4000–400 cm−1 revealed the characteristic peak values, intensity and functional groups present in the plant. The FTIR wavenumber frequencies of the crude aqueous-ethanolic extracts of the leaves, stem bark and root compared to corresponding raw dry plant parts confirmed the presence of alcohols, phenols, alkanes, esters, carboxylic acids, aromatics and possibly aliphatic amine compounds as major phytochemical constituents in the plant. The transmission, reflection and absorption data as a function of wavelength and absorption coefficient were determined by UV–Vis spectroscopy in solution and solid states ranging from 200–800 nm on a double beam Perkin Elmer spectrophotometer. Well resolved and separated characteristic broad absorption band peaks at the wavelength λmax 280–450 nm mostly associated with flavonoid and other phenolic compounds were obtained. In addition, a preliminary investigation into salt uptakes ability of the plant in predetermined concentrations of sodium chloride solution was carried out by pH and conductimetry methods. The report in this paper is the first communication on spectroscopic analysis of Sporobolus spicatus which is intended to provide useful information for further research on the value-added applications in biotechnology for the development of salt-tolerant crops for use in salt-afflicted agro-ecosystems or as a natural agent for wastewater purification and desalination.

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References

  1. FAO (2000) Global Network on Integrated Soil Management for Sustainable Use of Salt–Affected Soils. Rome, Italy. http://www.fao.org/ag/agl/agll/spush

  2. Hasanuzzaman M, Nahar K, Alam Md M, Bhowmik PC, Hossain Md A, Rahman MM, Prasad MNV, Ozturk M, Fujita M (2014) Potential use of halophytes to remediate saline soils. BioMed Res Int. Article ID 589341

    Google Scholar 

  3. Hasanuzzaman M, Nahar K, Fujita M (2013) Plant response to salt stress and role of exogenous protectants to mitigate salt-induced damages. In: Ahmad P, Azooz MM, Prasad MNV (eds) Eco physiology and responses of plants under salt stress, Springer, New York, USA, pp 25–87

    Google Scholar 

  4. Freedman A, Gross A, Shelef O, Rachmilevitch S, Arnon S (2014) Salt uptake and evapotranspiration under arid conditions in horizontal subsurface flow constructed wetland planted with halophytes. Ecol Eng 70:282–286

    Article  Google Scholar 

  5. Ashraf MY, Ashraf M, Sarwar G (2005) Physiological approaches to improving plant salt tolerance. In: Dris R (ed) Crops: growth, quality and biotechnology. WFL Publisher, Helsinki, Finland, pp 1206–1227

    Google Scholar 

  6. Rabhi M, Hafsi C, Lakhdar A (2009) Evaluation of the capacity of three halophytes to desalinize their rhizosphere as grown on saline soils under non-leaching conditions. Afr J Ecol 47:463–468

    Article  Google Scholar 

  7. Flowers TJ, Colmer TD (2008) Salinity tolerance in halophytes. New Phytol 179:945–963

    Article  CAS  PubMed  Google Scholar 

  8. Dracup MNH, Greenway H (1985) A procedure for isolating vacuoles from leaves of the halophyte Suaeda maritime. Plant, Cell Environ 8:149–154

    Article  Google Scholar 

  9. Naz N, Hameed M, Wahid A, Arshad M, Ahmad MSA (2009) Patterns of ion excretion and survival in two stoloniferous arid zone grasses. Physiol Plantarum 135:185–195

    Article  CAS  Google Scholar 

  10. Hameed A, Hussain T, Gulzar S, Aziz I, Gul B, Khan MA (2012) Salt tolerance of a cash crop halophyte Suaeda fruticosa: biochemical responses to salt and exogenous chemical treatments. Acta Physiol Plant 34:2331–2340

    Article  CAS  Google Scholar 

  11. Flower TJ, Hajibagheri MA, Clipson NJW (1986) Halophytes. Q Rev Biol 61:313–337

    Article  Google Scholar 

  12. Grigore MN, Ivanescu L, Toma C (2014) Halophytes: an integrative anatomical study. Springer International Publishing, Switzerland

    Google Scholar 

  13. Santos J, Al-azzawi M, Aronson J, Flower TJ (2015) eHALOPH a database of salt-tolerant plants: helping put halophytes to work. Plant Cell Physiol https://doi.org/10.1093/pcp/pcv155

    Article  PubMed  Google Scholar 

  14. Pouladi SF, Anderson BC, Wootton B, Rozema L (2016) Evaluation of phytodesalination potential of vegetated bioreactors treating greenhouse effluent. Water 8:233. https://doi.org/10.3390/w8060233

    Article  CAS  Google Scholar 

  15. Bartels D, Sukur R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24:23–58

    Article  CAS  Google Scholar 

  16. Bohnert HJ, Cushman JC (2000) The ice plant cometh: lessons in abiotic stress tolerance. J Plant Growth Regul 19:334–346

    Article  CAS  Google Scholar 

  17. Bohnert HJ, Nelson DE, Jensen RG (1995) Adaptations to environmental stresses. Plant Cell 7:1099–1111

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Rajalakshmi S, Parida A (2012) Halophytes as a source of genes for abiotic stress tolerance. J Plant Biochem Biotechnol 21:S63–S67

    Article  Google Scholar 

  19. Kane NC, Rieseberg LH (2007) Selective sweeps reveal candidate genes for adaptation to drought and salt tolerance in common sunflower Helianthus annuus. Genetics 175:1823–1834

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Lowry DB, Hall MC, Salt DE, Willis JH (2009) Genetic and physiological basis of adaptive salt tolerance divergence between coastal and inland Mimulus guttatus. New Phytol 183:776–788

    Article  PubMed  Google Scholar 

  21. Agarwal PK, Shukla PS, Gupta K, Jha B (2013) Bioengineering for salinity tolerance in plants: state of the art. Mol Biotechnol 54:102–123

    Article  CAS  PubMed  Google Scholar 

  22. Shabala S, Mackay A (2011) Ion transport in halophytes. Adv Bot Res 57:151–199

    Article  CAS  Google Scholar 

  23. Flowers TJ, Troke PF, Yeo AR (1977) The mechanism of salt tolerance in halophytes. Ann Rev Plant Physio 28:89–121

    Article  CAS  Google Scholar 

  24. Teakle NL, Tyerman SD (2010) Mechanisms of Cl− transport contributing to salt tolerance. Plant, Cell Environ 33:566–589

    Article  CAS  Google Scholar 

  25. Apse MP, Blumwald E (2007) Na+ transport in plants. FEBS Lett 581:2247–2254

    Article  CAS  PubMed  Google Scholar 

  26. Rodriguez-Rosales MP, Galvez FJ, Huertas R, Aranda MN, Baghour M, Cagnac O, Venema K (2009) Plant NHX cation/proton antiporters. Plant Signaling and Behaviour 4:265–276

    Article  CAS  Google Scholar 

  27. Burkill HM (1985) The useful plants of west tropical Africa. Royal Botanic Gardens Kew, vol 2

    Google Scholar 

  28. Odebiyi OO, Sofowora EA (1978) Phytochemical screening of Nigeria medical plants II. Lloydia 41:234–246

    CAS  PubMed  Google Scholar 

  29. Coates J (2000) Interpretation of infrared spectra, a practical approach. In: Meyers RA (ed) Encyclopedia of analytical chemistry. Wiley, Chichester, UK, pp 10815–10837

    Google Scholar 

  30. Adeloye AO (2011) Synthesis, photophysical and electrochemical properties of a mixed bipyridyl-phenanthrolyl ligand Ru(II) heteroleptic complex having trans-2-methyl-2-butenoic acid functionalities. Molecules 16:8353–8367

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Markham KR (1982) Techniques of flavonoid identification (1st edn). Academic Press, UK, ISBN 978-0124726802

    Google Scholar 

  32. Petro FP, Goncalo CJ (2012) Structural analysis of flavonoids and related compounds—a review of spectroscopic applications. Centro de Quimica Estrutural 2:33–54

    Google Scholar 

  33. Cea P, Martin S, Villares A, Mobius D, Carmen Lopez M (2005) Use of UV–Vis reflection spectroscopy for determining the organization of viologen and viologen tetracyanoquinodimethanide monolayers. J Phys Chem B 110:963–970

    Article  Google Scholar 

  34. Upasani SM, Kotkar HM, Mendki PS, Maheshwari VL (2003) Partial characterization and insecticidal properties of Ricinus communis L foliage flavonoids. Pest Manag Sci 59:1349–1354

    Article  CAS  PubMed  Google Scholar 

  35. Ponce SC, Prado C, Pagano E, Prado FE, Rosa M (2015) Effect of solution pH on the dynamic of biosorption of Cr(VI) by living plants of Salvinia minima. Ecol Eng 74:33–41

    Article  Google Scholar 

  36. Kulkarni RM, Shetty KV, Srinikethan G (2013) Cd(II) and Ni(II) biosorption by Basillus katerosporus (MTCC 1628). J Taiwan Inst Chem E 45:1628–1635

    Article  Google Scholar 

  37. Prabhu SG, Salian CS, Kiran SK, Naik MM, Srinikethan G (2016) Studies on the potential of Adiantum philippense L. as a biosorbent for nickel removal. Res J Chem Environ Sci 4:31–39

    Google Scholar 

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Acknowledgements

The author wishes to express appreciations to Prof. Oluwatoyin Kolawole and Mr Joseph Madome of the Okavango Research Institute, Maun, Botswana for the collection and identification of the plant.

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Authors and Affiliations

  1. Faculty of Applied Sciences, Department of Chemistry, Cape Peninsula University of Technology, Bellville Campus, 7535, Cape Town, Republic of South Africa

    Adewale Olufunsho Adeloye

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  1. Adewale Olufunsho Adeloye
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Correspondence to Adewale Olufunsho Adeloye .

Editor information

Editors and Affiliations

  1. Department of Chemistry, Faculty of Science, University of Mauritius, Reduit, Mauritius

    Ponnadurai Ramasami

  2. Dept of Chemistry, Faculty of Scien, University of Mauritius, Reduit, Mauritius

    Minu Gupta Bhowon

  3. Dept. of Chemistry, Faculty of Scie, University of Mauritius, Reduit, Mauritius

    Sabina Jhaumeer Laulloo

  4. Dept. of Chem., Fac. of Science, University of Mauritius, Reduit, Mauritius

    Henri Li Kam Wah

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Adeloye, A.O. (2019). Conductimetric Analysis and Preliminary Evaluation of Phytochemical Constituents of Sporobolus spicatus (Vahl) Kunth [Family: Poaceae] by FTIR and UV–Vis Spectroscopic Techniques. In: Ramasami, P., Gupta Bhowon, M., Jhaumeer Laulloo, S., Li Kam Wah, H. (eds) Chemistry for a Clean and Healthy Planet. ICPAC 2018. Springer, Cham. https://doi.org/10.1007/978-3-030-20283-5_3

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