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Structural characterization of n-hexadecanoic acid from the leaves of Ipomoea eriocarpa and its antioxidant and antibacterial activities

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Abstract

The present study was to evaluate the antioxidant and antibacterial activity of n-hexadecanoic acid from the leaves of Ipomoea eriocarpa through aqueous ethanol extraction and further purification by column chromatography. The structure characterization of the isolated compound was elucidated by FTIR, ESI–MS, 1H-NMR, and 13C-NMR spectroscopy. The antioxidant activities of n-hexadecanoic acid were as follows: DPPH (30.19–89.13% at 100–500 µg/ml), ABTS (42.18–83.86% at 100–500 µg/ml), reducing power (0.02–0.16% at 100–500 µg/ml), nitric oxide (18.65–73.17% at 100–500 µg/ml), and superoxide (17.18–81.21% at 100–500 µg/ml) respectively. The n-hexadecanoic acid showed that moderate antibacterial activity against S. aureus, B. subtilis, E. coli, and K. pneumoniae was 7.96 \(\pm\) 0.05 mm, 10.96 \(\pm\) 0.15 mm, 11.10 \(\pm\) 0.17 mm, and 11.93 \(\pm\) 0.11 mm at the maximum concentration of 50 µg/ml. Hence, the good biological activity of the n-hexadecanoic acid from I. eriocarpa could be used as a natural antioxidant and antibacterial agent in the future pharmaceutical industry.

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The data used to support the finding of this study are included within the manuscript.

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Acknowledgements

The author (T.G) is grateful to Periyar University, India, for providing a university research fellowship.

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Author notes

  1. Murugesan Subban and Palaniappan Seedevi have equal corresponding contribution.

Authors and Affiliations

  1. Department of Botany, Periyar University, Periyar Palkalai Nagar, Salem, 636011, Tamil Nadu, India

    Theivanai Ganesan & Murugesan Subban

  2. Department of Chemistry, Periyar University, Periyar Palkalai Nagar, Salem, 636011, Tamil Nadu, India

    Denzil Britto Christopher Leslee & Shanmuga Bharathi Kuppannan

  3. Department of Research and Innovation, Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai - 602105, Tamil Nadu, India

    Palaniappan Seedevi

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Contributions

Theivanai Ganesan: extraction, purification and biological activity evaluation in whole manuscript; Murugesan Subban: supervised the work and guidance; Denzil Britto Christopher Leslee: NMR analysis; Shanmuga Bharathi Kuppannan: NMR interpretation; Palaniappan Seedevi: biological and chemical analysis.

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Correspondence to Palaniappan Seedevi.

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Ganesan, T., Subban, M., Christopher Leslee, D.B. et al. Structural characterization of n-hexadecanoic acid from the leaves of Ipomoea eriocarpa and its antioxidant and antibacterial activities. Biomass Conv. Bioref. (2022). https://doi.org/10.1007/s13399-022-03576-w

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