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The role of enzyme amylase in two germinating seed morphs of Halopyrum mucronatum (L.) Stapf. in saline and non-saline environment

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Abstract

The role of enzyme amylase in two germinating seed morphs, i.e. black and brown, of Halopyrum mucronatum in saline and non-saline environment was examined. Both seed morphs of this halophytic grass have variations in their moisture content, total lipid, protein, sugar, phenol and tannin contents. Black seed exhibited higher activity compared to brown in saline medium. Sugar mobilization in both seed morphs was also affected due to the difference in amylase activity. However, exogenous application of GA3 in saline medium enhanced the amylase activity and sugar mobilization. Phenolic contents were similar except for vanillic acid which was found only in brown seeds while catechol was present only in black seeds. Phenols extracted from both seed morphs were applied to determine their effects on amylase activity. Phenolic extracts obtained from brown seeds showed higher degree of inhibition of amylase activity. Results are discussed in relation to seed coat phenols, leaching, amylase activity and sugar mobilization.

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Acknowledgments

ZSS is thankful to Dean, Faculty of Science, University of Karachi who provided financial assistance for research works.

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Authors and Affiliations

  1. Institute of Sustainable Halophyte Utilization, University of Karachi, Karachi, Pakistan

    M. Ajmal Khan

  2. Department of Botany, University of Karachi, Karachi, Pakistan

    Zamin Shaheed Siddiqui

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  1. Zamin Shaheed Siddiqui
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  2. M. Ajmal Khan
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Correspondence to Zamin Shaheed Siddiqui.

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Communicated by J. Zwiazek.

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Siddiqui, Z.S., Khan, M.A. The role of enzyme amylase in two germinating seed morphs of Halopyrum mucronatum (L.) Stapf. in saline and non-saline environment. Acta Physiol Plant 33, 1185–1197 (2011). https://doi.org/10.1007/s11738-010-0646-z

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  • DOI: https://doi.org/10.1007/s11738-010-0646-z

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