Abstract
Eucalyptus are trees sensitive to interference imposed by weeds mainly during the early growth phase. The repetitive use of herbicides with the same mechanism of action has led to the selection of resistant weeds, and Digitaria insularis stands out for its resistance to glyphosate. Due to its occurrence in eucalyptus plantations, knowledge regarding D. insularis interference in eucalyptus early growth is needed to develop management strategies. This study aimed to confirm D. insularis glyphosate-resistence; and also to evaluate the effect of densities and distances of glyphosate-resistant D. insularis, seeded and in regrowth, on the early growth of Eucalyptus urophylla × E. grandis (Clone AEC-144). Prior to eucalyptus’ experiments, D. insularis glyphosate-resistance was confirmed through a dose–response curve experiment. After that, two experiments [one for weed’s first growth (seeded) and other for second growth (regrowth)] were carried out in an open area, in 700 L pots, for 105 days after planting eucalyptus seedlings. The treatments consisted of increasing densities of D. insularis (0, 1, 2 and 3 plants m−2) planted at 10, 20 or 30 cm from the eucalyptus seedlings. Eucalyptus height, stem diameter, leaf area and dry matter were evaluated. The eucalyptus variables most sensitive to interference were leaf and stem dry matter, with reductions of 45% and 88.4%, respectively for weeds seeded and regrowth. Weed second growth (regrowth) interfered more aggressively in eucalyptus growth. The coexistence with D. insularis, regardless of density and distance, negatively interfered early tree growth.
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Adams P, Beadle C, Mendham N, Smethurst P (2003) The impact of timing and duration of grass control on the growth of a young Eucalyptus globulus Labill. plantation. New for 26:147–165. https://doi.org/10.1023/A:1024490707175
ALAM – Asociación Latinoamericana De Malezas (1974) Recomendaciones sobre unificación de los sistemas de evaluación en ensayos de control de malezas. Asociación Latinoamericana De Malezas 1:35–38
Bacha AL, Martins PFRB, Alves PLCA, Paula RC (2018) Trinexapac-ethyl causes stimulatory effect on eucalyptus initial growth under nutritional deficiency. Can J for Res 48(1):94–100. https://doi.org/10.1139/cjfr-2017-0245
Bacha AL, Pereira FCM, Pires RN, Nepomuceno MP, Alves PLCA (2016) Interference of seeding and regrowth of signal grass weed (Urochloa decumbens) during the initial development of Eucalyptus urograndis (E. grandis × E. urophylla). Aust J Crop Sci. 10(3):322–330. https://doi.org/10.21475/ajcs.2016.10.03.p6995
Barroso AAM, Michelon TB, Alves PLCA, Han H, Yu Q, Powles SB, Vila-Aiub MM (2022) Challenging glyphosate resistance EPSPS P106S and TIPS mutations with soybean competition and glyphosate: implications for management. Pest Manag Sci 78(11):4764–4773. https://doi.org/10.1002/ps.7096
Barroso GM, da Silva RS, Mucida DP, Borges CE, Ferreira SR, dos Santos JCB, Lins HA, Mendonça V, Silva DV, dos Santos JB (2021) Spatio-temporal distribution of Digitaria insularis: risk analysis of areas with potential for selection of glyphosate-resistant biotypes in eucalyptus crops in Brazil. Sustainability 13:10405. https://doi.org/10.3390/su131810405
Blackshaw RE, Harker KN (2002) Selective weed control with glyphosate in glyphosate-resistant spring wheat (Triticum aestivum). Weed Technol 16:885–892. https://doi.org/10.1614/0890-037X(2002)016[0885:SWCWGI]2.0.CO;2
Bleasdale JKA (1960) Studies on plant competition. In: Harper JL (ed) The biology of weeds. Oxford, pp 133–142
Bleiholder H, Kirfel H, Langelüddecke P, Stauss R (1991) Codificação unificada dos estádios fenológicos de culturas e ervas daninhas. Pesq Agropec Bras 26(9):1423–1429
Braga AF, Barroso AAM, Amaral CL, Nepomuceno MP, Alves PLCA (2018) Population interference of glyphosate resistant and susceptible ryegrass on eucalyptus initial development. Planta Daninha 36:e018170148. https://doi.org/10.1590/S0100-83582018360100086
Caldwell J, Sucoff E, Dixon K (1995) Grass interference limits resource availability and reduces growth of juvenile red pine in the field. New for 10:1–15. https://doi.org/10.1007/BF00034173
Carvalho LB, Cruz-Hipolito H, González-Torralva F, Alves PLCA, Christoffoleti PJ, Prado R (2011) Detection of sourgrass (Digitaria insularis) biotypes resistant to glyphosate in Brazil. Weed Sci 59(2):171–176. https://doi.org/10.1614/WS-D-10-00113.1
Cerdeira AL, Gazziero DLP, Duke SO, Matallo MB (2011) Agricultural impacts of glyphosate-resistant soybean cultivation in South America. J Agric Food Chem 59(11):5799–5807. https://doi.org/10.1021/jf102652y
Cesarin AE, Martins PFRB, Barroso AAM, Carrega WC, Alves PLCA, Bianco S (2019) Dry matter and macronutrient accumulation in Digitaria insularis biotypes susceptible and resistant to glyphosate. J Agric Sci 11(4):151–159. https://doi.org/10.5539/jas.v11n4p151
Chappelle EW, Kim MS (1992) Ratio analysis of reflectance spectra (RARS): an algorithm for the remote estimation of the concentrations of chlorophyll a, chlorophyll b, and carotenoids in soybean leaves. Remote Sens Environ 39(3):239–247. https://doi.org/10.1016/0034-4257(92)90089-3
Christoffoleti PJ, López-Ovejero R (2003) Main aspects on weed herbicide resistance to glyphosate. Planta Daninha 21(3):507–515. https://doi.org/10.1590/S0100-83582003000300020
Clapp J (2021) Explaining growing glyphosate use: the political economy of herbicide-dependent agriculture. Glob Environ Chang 67:102239. https://doi.org/10.1016/j.gloenvcha.2021.102239
Coll L, Balandier P, Picon-Cochard C (2004) Morphological and physiological responses of beech (Fagus sylvatica) seedlings to grass-induced belowground competition. Tree Physiol 24(1):45–54. https://doi.org/10.1093/treephys/24.1.45
Colmanetti MAA, Bacha AL, Alves PLCA, Paula RC (2019) Effect of increasing densities of Urochloa brizantha cv. Marandu on Eucalyptus urograndis initial development in silvopastoral system. J for Res 30:537–543. https://doi.org/10.1007/s11676-018-0635-4
Colmanetti MAA, Bacha AL, Braga AF, Alves PLCA, Paula RC (2017) Interference of Urochloa decumbens and Panicum maximum in the initial growth of six clones of Eucalyptus urograndis. Aust J Crop Sci 11(10):1261–1267. https://doi.org/10.21475/ajcs.17.11.10.pne514
Costa AGF, Bacha AL, Pires RN, Pavani MCMD, Alves PLCA (2021) Interference of Commelina benghalensis in the initial growth of Eucalyptus grandis in winter and summer. Ciência Florestal 31(2):590–606. https://doi.org/10.5902/1980509825556
Cruz MB, Alves PLCA, Karam D, Ferraudo AS (2010) Capim-colonião e seus efeitos sobre o crescimento inicial de clones de Eucalyptus × urograndis. Ciência Florestal 20(3):391–401. https://doi.org/10.5902/198050982054
Dinardo W, Toledo REB, Alves PLCA, Pitelli RA (2003) Efeito da densidade de plantas de Panicum maximum. Jacq. Sobre o crescimento inicial de Eucalyptus grandis W. Hill ex Maiden. Scientia Forestalis 64:59–68
Donahue RA, Berg VS, Vogelmann TC (1990) Assessment of the potential of the blue light gradient in soybean pulvini as a leaf orientation signal. Physiol Plant 79(4):593–598. https://doi.org/10.1111/j.1399-3054.1990.tb00030.x
Fisher RA (1925) Statistical methods for research workers. Oliver & Boyd, Edinburgh
Florentine S, Fox J (2003) Competition between Eucalyptus victrix seedlings and grass species. Ecol Res 18:25–39. https://doi.org/10.1046/j.1440-1703.2003.00531.x
Galvan-Cisneros CM, Gastauer M, Massante C, Villa PM, Meira-Neto JAA (2023) Simultaneous competition and environmental filtering in woody communities of the understory of Eucalyptus plantations in the Cerrado. Perspect Plant Ecol Evol Syst 59:12573. https://doi.org/10.1016/j.ppees.2023.125731
Garau AM, Ghersa CM, Lemcoff JH, Barañao JJ (2009) Weeds in Eucalyptus globulus subsp. maidenii (F. Muell) establishment: effects of competition on sapling growth and survivorship. New for 37:251–264. https://doi.org/10.1007/s11056-008-9121-8
Garau AM, Lemcoff JH, Ghersa CM, Beadle CL (2008) Water stress tolerance in Eucalyptus globulus Labill. subsp. maidenii (F. Muell.) saplings induced by water restrictions imposed by weeds. Forest Ecol Manag 255:2811–2819. https://doi.org/10.1016/j.foreco.2008.01.054
Gonçalves JLM, Alvares CA, Higa AR, Silva LD, Alfenas AC, Stahl J, Ferraz SFB, Lima WP, Brancalion PHS, Hubner A, Bouillet JPD, Laclau JP, Nouvellon Y, Epron D (2013) Integrating genetic and silvicultural strategies to minimize abiotic and biotic constraints in Brazilian eucalyptus plantations. For Ecol Manag 301:6–27. https://doi.org/10.1016/j.foreco.2012.12.030
Graat Y, Bacha AL, Nepomuceno MP, Alves PLCA (2018) Initial development of eucalyptus according to different desiccation periods of signalgrass. Planta Daninha 2018(v36):e018145168. https://doi.org/10.1590/S0100-83582018360100020
Graat Y, Rosa JO, Nepomuceno MP, Carvalho LB, Alves PLCA (2015) Grass weeds interfering with eucalyptus: effects of the distance of coexistence on the initial plant growth. Planta Daninha 33:203–211. https://doi.org/10.1590/0100-83582015000200005
Grossnickle S, Folk R (1993) Stock quality assessment: forecasting survival and performance on a reforestation niche. Tree Plant Notes 44(3):113–121
Heap I (2023) The international survey of herbicide resistant weeds. www.weedscience.com. Accessed 5 March 2023
Heap I, Duke SO (2018) Overview of glyphosate-resistant weeds worldwide. Pest Manag Sci 74:1040–1049. https://doi.org/10.1002/ps.4760
Hébert MP, Fugère V, Gonzalez A (2019) The overlooked impact of rising glyphosate use on phosphorus loading in agricultural watersheds. Front Ecol Environ 17(1):48–56. https://doi.org/10.1002/fee.1985
Huang Z, Xu Z, Blumfield TJ, Bubb K (2008) Variations in relative stomatal and biochemical limitations to photosynthesis in a young blackbutt (Eucalyptus pilularis) plantation subjected to different weed control regimes. Tree Physiol 28:997–1005. https://doi.org/10.1093/treephys/28.7.997
Hunt MA, Battaglia M, Davidson NJ, Unwin GL (2006a) Competition between plantation Eucalyptus nitens and Acacia dealbata weeds in northeastern Tasmania. Forest Ecol Manag 233:260–274. https://doi.org/10.1016/j.foreco.2006.05.017
Hunt MA, Battaglia M, Davidson NJ, Unwin GL (2006b) Competition between plantation Eucalyptus nitens and Acacia dealbata weeds in northeastern Tasmania. For Ecol Manag 233:260–274. https://doi.org/10.1016/j.foreco.2006.05.017
Ibá (2022) Indústria brasileira de árvores. Relatório Ibá. https://iba.org/datafiles/publicacoes/relatorios/relatorio-anual-iba2022-compactado.pdf. Accessed 5 March 2023
Köppen W (1948) Climatologia: con un estudio de los climas de la tierra. Fondo de Cultura Econômica, México
Lamhamedi M, Bernier P, Hebert C, Jobidon R (1998) Physiological and growth responses of three sizes of containerized Picea mariana seedlings outplanted with and without vegetation control. For Ecol Manag 110:13–23
Little KM, Rolando CA, Morris CD (2007) An integrated analysis of 33 Eucalyptus trials linking the onset of competition-induced tree growth suppression with management, physiographic and climatic factors. Ann for Sci 64:585–591. https://doi.org/10.1051/forest:2007036
Little KM, Staden JV (2005) Effects of vegetation control on Eucalyptus grandis × E. camaldulensis volume and economics. S Afr J Bot 71:418–425
Ma L, Upadhyaya MK (2017) Effects of red/far-red light ratio on common lamb’s-quarters, redroot pigweed, and tomato plants. Can J Plant Sci 97(3):494–500. https://doi.org/10.1139/cjps-2016-0089
Netto AG, Cordeiro EM, Nicolai M, Carvalho SJ, Ovejero RFL, Brunharo CA, Zucchi MI, Christoffoleti PJ (2021) Population genomics of Digitaria insularis from soybean areas in Brazil. Pest Manag Sci 77:5375–5381. https://doi.org/10.1002/ps.6577
Pavan BE, Amaral RG, Pupin S, Costa RML, Dias DC, Scarpinati EA, Paula RC (2021) Competitive ability among Eucalyptus spp. commercial clones in Mato Grosso do Sul state. Forest Ecol Manag 494:119297. https://doi.org/10.1016/j.foreco.2021.119297
Pereira FCM, Alves PLCA, Martins JVF (2013) Interference of grasses on the growth of eucalyptus clones. J Agric Sci 5:173–180. https://doi.org/10.5539/jas.v5n11p173
Pereira FCM, Yamauti MS, Alves PLCA (2012) Interaction between weed management and covering fertilization in the initial growth of Eucalyptus grandis x E. urophylla. Revista Árvore 36:941–950. https://doi.org/10.1590/S0100-67622012000500016
Pitelli RA (1985) Interferência das plantas daninhas em culturas agrícolas. Informe Agropecuário 11(129):16–27
Pitelli RA, Marchi SR (1991) Interferência das plantas invasoras nas áreas de reflorestamento. III Seminário técnico sobre plantas daninhas e o uso de herbicidas em reflorestamento, Belo Horizonte, pp 1–11
Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta Bioenerg 975(3):384–394. https://doi.org/10.1016/S0005-2728(89)80347-0
Powles SB, Yu Q (2010) Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol 61:317–347. https://doi.org/10.1146/annurev-arplant-042809-112119
Qin F, Liu S, Yu S (2018) Effects of allelopathy and competition for water and nutrients on survival and growth of tree species in Eucalyptus urophylla plantations. For Ecol Manag 424:387–395. https://doi.org/10.1016/j.foreco.2018.05.017
Resende RT, Marcatti GE, Pinto DS, Takahashi EK, Cruz CD, Resende MDV (2016) Intra-genotypic competition of Eucalyptus clones generated by environmental heterogeneity can optimize productivity in forest stands. For Ecol Manag 15:50–58. https://doi.org/10.1016/j.foreco.2016.08.041
Ripullone F, Grassi G, Lauteri M, Borghetti M (2003) Photosynthesis–nitrogen relationships: interpretation of different patterns between Pseudotsuga menziesii and Populus× euroamericana in a mini-stand experiment. Tree Physiol 23(2):137–144. https://doi.org/10.1093/treephys/23.2.137
Sands R, Nambiar EKS (1984) Water relations of Pinus radiata in competition with weeds. Can J Forest Res 14:233–237. https://doi.org/10.1139/x84-045
Santos MV, Ferreira EA, Tuffi Santos LD, da Fonseca DM, Aspiazu I, Silva DV, Porto JMP, Braga RR (2015) Physiological aspects of acacia and eucalyptus in competition with Brachiaria. Aust J Crop Sci 9(3):210–214
Schaller M, Schroth G, Beer J, Jimenez F (2003) Root interactions between young Eucalyptus deglupta trees and competitive grass species in contour strips. For Ecol Manage 179:429–440. https://doi.org/10.1016/S0378-1127(02)00534-0
Sharkey TD, Raschke K (1981) Effect of light quality on stomatal opening in leaves of Xanthium strumarium L. Plant Physiol 68(5):1170–1174. https://doi.org/10.1104/pp.68.5.1170
Silva JRV, Alves PLCA, Toledo REB (2012) Weed control strip influences the initial growth of Eucalyptus grandis. Acta Sci-Agron 34(1):29–35. https://doi.org/10.4025/actasciagron.v34i1.12252
Sneath PH, Sokal RR (1973) Numerical taxonomy: the principles and practice of numerical classification. Freeman, San Francisco, WH
Stape JL, Binkley D, Ryan MG, Gomes AND (2004) Water use, water limitation, and water use efficiency in a Eucalyptus plantation. Bosque 25(2):35–41. https://doi.org/10.4067/S0717-92002004000200004
Tarouco CP, Agostinetto D, Panozzo LE, Santos LS, Vignolo GK, Ramos LOO (2009) Weed interference periods on in the initial growth of eucalyptus. Pesq Agrop Brasileira 44(9):1131–1137. https://doi.org/10.1590/S0100-204X2009000900010
Tiburcio RAS, Bacha AL, Alves PLCA, Salgado TP (2023) Long-term growth response to weed-control strips in Eucalyptus urograndis plantations in Brazil. Aust for 86(1):22–30. https://doi.org/10.1080/00049158.2023.2167155
Toledo REB, Victória Filho R, Alves PLCA, Pitelli RA, Cadini MTD (2000) Effects of Brachiaria decumbens strip control on eucalyptus initial plants development. Planta Daninha 18(3):383–393. https://doi.org/10.1590/S0100-83582000000300001
Toledo REB, Victória Filho R, Alves PLCA, Pitelli RA, Cadini MTD (2001) Efeito da densidade de plantas de Brachiaria decumbens Stapf. sobre o crescimento inicial de mudas de Eucalyptus grandis W. Hill ex Maiden. Scientia Forestalis 60:109–117
Vila-Aiub MM, Gundel PE, Preston C (2015) Experimental methods for estimation of plant fitness costs associated with herbicide-resistance genes. Weed Sci 63:203–216. https://doi.org/10.1614/WS-D-14-00062.1
Vila-Aiub MM, Neve P, Roux F (2011) A unified approach to the estimation and interpretation of resistance costs in plants. Heredity 107(5):386–394. https://doi.org/10.1038/hdy.2011.29
Watt MS, Whitehead D, Mason EG, Richardson B, Kimberley MO (2003) The influence of weed competition for light and water on growth and dry matter partitioning of young Pinus radiata, at a dryland site. Forest Ecol Manag 183:363–376. https://doi.org/10.1016/S0378-1127(03)00139-7
Yanniccari M, Vila-Aiub M, Istilart C, Acciaresi H, Castro AM (2016) Glyphosate resistance in perennial ryegrass (Lolium perenne L.) is associated with a fitness penalty. Weed Sci 64(1):71–79. https://doi.org/10.1614/WS-D-15-00065.1
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The authors are grateful for the technical support provided by Weed Sciences Laboratory (LAPDA) and also to the financial support provided by São Paulo Research Foundation (FAPESP) (grant #20/06162-6) to the first author.
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São Paulo Research Foundation (FAPESP) (grant #20/06162-6).
Fundação de Amparo à Pesquisa do Estado de São Paulo, 20/06162-6
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Study conception and design: T.B.G., A.L.B. and P.L.C.A.A.; Methodology implementation, experiment execution and data collection: T.B.G. and A.L.B.; Data analysis/interpretation: T.B.G., A.L.B. and P.L.C.A.A.; Manuscript writing/revision: T.B.G., A.L.B., H.L.M., B.D.N. and P.L.C.A.A.
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Guidugli, T.B., Bacha, A.L., Martins, H.L. et al. Glyphosate-resistant Digitaria insularis effect on eucalyptus initial growth. New Forests (2024). https://doi.org/10.1007/s11056-024-10028-y
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DOI: https://doi.org/10.1007/s11056-024-10028-y