Abstract
Two different but related sequences that encode Vigna luteola 7S vicilins were isolated and characterized. The sequences differ by two nucleotide substitutions, each of which results in an amino acid replacement. This low level of divergence suggests that a recent gene duplication has occurred. Both variants are expressed in cDNA populations; therefore, neither gene is a pseudogene. Both copies were present in all individuals (72) analyzed using real-time PCR and TaqMan probes. Segregation was not observed. The two sequences are not independent alleles. Vicilin genomic sequences of 11 specimens from six geographic locations were determined. No polymorphic sites were identified in either of the two gene copies. This lack of polymorphism suggests that either a population bottleneck or selection has occurred. The genetic structure, expression patterns, and protein composition of the V. luteola vicilins were compared to those of other legume vicilins.
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Acknowledgments
We thank Ms. Angela Geraci and Mr. Vijay Chigarapati for critical reading of this manuscript. We thank Dr. Joel Stake and Dr. Brian Mahon for assistance in collection of field samples. This study was supported by a State of Louisiana Board of Regents Support Fund Research and Development Grant LEQSF (2002-04)-RD-A-32.
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Xie, Z., Neigel, J. & Chlan, C. Vicilin Genes of Vigna luteola: Structure, Organization, Expression, and Variation. Biochem Genet 50, 372–388 (2012). https://doi.org/10.1007/s10528-011-9481-1
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DOI: https://doi.org/10.1007/s10528-011-9481-1