Abstract
Four submerged macrophytes were analysed with their respective assemblages of chironomid and algal taxa. Studies were made bimonthly, from January to November, in a shallow macrophyte-dominated lake. The periphytic algal community was represented by chlorophytes, diatoms and cyanobacteria; diatoms showed the highest relative abundance on the macrophyte species during the study. Algal biomass was affected by the season, and in January the highest values were found on Stratiotes aloides L., Potamogeton lucens L., Myriophyllum spicatum L.; in July on Chara aculeolata Kütz. The lowest algal biomass, on most of the macrophytes studied, was found in September. Larvae of Dicrotendipes sp., Diplocladius cultriger Kieffer, Endochironomus albipennis gr., Endochironomus impar (Walker), Glyptotendipes sp. and Paratanytarsus austriacus Kieffer were dominant within the chironomid community. The spatial distribution of chironomids between macrophytes species was related to periphytic algae biomass and certain environmental variables (temperature, water transparency, biomass of chlorophytes and diatoms). Analysis of chironomid diet and the results of canonical correspondence analysis confirmed this relationship. The strongest chironomid-algae relationships were observed on S. aloides and P. lucens.
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Introduction
In lakes whose water is clear, species and morphological diversity of submerged macrophytes may affect the distribution and structure of epiphytic invertebrates. Macrophytes exhibit variations in structural architecture and determine the habitat area available for colonisation by invertebrates (Cyr and Downing 1988; van de Meutter et al. 2008). Plants with dissected leaves (such as Ceratophyllum demersum, Myriophyllum spicatum) are often more complex, owing to the high ratio of surface area to plant mass; thus it has been suggested this complexity increases the number of microhabitats and overall niche space for associated invertebrates (Warfe and Barmuta 2004; Hansen et al. 2011). The distribution of invertebrates between macrophyte species is also related to potential food resources (periphytic algae). Many groups of invertebrate grazers use periphytic algae as a major food resource; one of the most important is chironomid larvae (Peets et al. 1994; Balci and Kennedy 2003; Tarkowska-Kukuryk 2011). Although the larvae are able to ingest a variety of food types, including algae, detritus and associated microorganisms and macrophytes (Pinder 1986; Hirabayashi and Wotton 1999), commonly reported food items are periphytic algae (Lawrence and Gresens 2004; Tall et al. 2006; Maasri et al. 2010).
High chironomid grazing pressure may substantially reduce the biomass of periphytic algae (Jones and Sayer 2003; Tarkowska-Kukuryk 2013). For this reason, chironomid larvae may act as an important top-down regulator of periphyton biomass, and the removal of periphyton by grazing larvae may improve light conditions for macrophytes. Periphytic algae may attenuate most of the light reaching the adaxial leaf surface, and decrease the production of macrophyte species markedly (up to 60–80 %). Thus, periphyton is an important competitor of macrophytes for light, carbon and nutrients, and may negatively affect the growth of the hosting macrophyte (Vermaat and Hootsmans 1994; Liboriussen et al. 2005). Accumulation of periphytic algae is usually greater on thick and finely branched submerged plants such as Myriophyllum or Ranunculus than on large-leaved pondweeds (Potamogeton) (Blindow 1987; Laugaste and Reunanen 2005). Understanding the algae-chironomid relationship of different macrophyte species requires an analysis of chironomid diet.
The colonisation of macrophyte species by periphytic algae and chironomids may also be stimulated by fish. Intensive fish foraging may substantially reduce the number of grazers (Brönmark et al. 1992; McCollum et al. 1988). The refuge effect of macrophytes is particularly evident in clear-water lakes, and depends on the macrophyte species. Koperski (1998) and Rantala et al. (2004) have stressed the role of the macrophyte species Stratiotes aloides as an effective shelter against predator pressure; similarly, dense characean stands may prevent intensive fish foraging (James et al. 2000). These highly developed green algae tend to cover the sediment in dense mats which resemble meadows.
To date a number of field and laboratory studies have focused on the relationships between plant morphology (substrate surface, habitat complexity) and associated invertebrates (Lalonde and Dowing 1991; Taniguchi et al. 2003; Tessier et al. 2004; Becerra-Munoz and Schramm 2007; Hansen et al. 2011). Only a few considered the structure and biomass of periphytic algae as a factor potentially determining the selection of macrophyte species by invertebrates (Dvorak 1996; Cattaneo et al. 1998). The significant contribution of environmental variables on chironomid variability is already known. Brooks et al. (2001) showed that chironomid assemblages in lakes of the north-west of England are structured by total phosphorus (TP), dissolved oxygen, lake depth, Secchi disc transparency and water temperature; in polluted water-bodies, the most important variables are dissolved oxygen and food availability (Lindegaard 1995). The chironomids associated with the common reed (Phragmites australis (Cav.) Trin. ex Steud.) are mostly affected by nutrients, epiphytic chlorophyll-a, periphyton biomass, reed density and total organic carbon (Cañedo-Argüelles and Rieradevall 2009; Tarkowska-Kukuryk 2011). Nevertheless, the determinants governing spatial distribution of chironomid species between macrophytes in clear-water lakes are still not well recognised.
During the study the following hypotheses were tested: that in a macrophyte-dominated lake: (1) the morphological structure of macrophytes determines the colonisation area and availability of food resources (periphytic algae) for chironomids; (2) the distribution of chironomids between macrophyte species is affected by the macrophyte species and the composition of periphytic algae; and (3) the relationships between macrophytes and associated chironomid larvae are affected by environmental variables (water temperature, Secchi disc transparency, oxygen content, nutrients).
The general objectives of the study were as follows: (1) to describe the taxonomic composition and abundance of periphytic algae and of chironomid larvae on four macrophyte species with different morphological structures; (2) to identify algal taxa in the guts of the dominant chironomid taxa and to compare them with the composition of the periphytic algae; and (3) to determine the environmental (habitat) conditions affecting the distribution of chironomids between macrophyte species.
Methods
Lake Skomielno
Lake Skomielno is a shallow, macrophyte-dominated lake situated in Polesie Lubelskie (eastern Poland) (51°29′N, 23°0′E, max. depth 6.5 m, surface area 75 ha). The lake is slightly eutrophic; the water is well oxygenated and transparent. Secchi disc transparency often reached the bottom, except in the deepest part of the lake. The bottom area overgrown by vegetation exceeded 67 %.
The study sites were situated inside beds of Chara aculeolata (site 1; north-eastern part of the lake; sampling depth 1.5 m), Stratiotes aloides (site 2; southern part of the lake; sampling depth 0.9 m), Potamogeton lucens (site 3; south-western part of the lake; sampling depth 1.2 m) and Myriophyllum spicatum (site 4; south-eastern part of the lake; depth 0.8 m). Samples of macrophytes, periphytic algae and chironomids were collected simultaneously, bimonthly from January to November during 2008 and 2009, assuming that every month is a season. Three replicates were collected each sampling month and from each site.
Macrophyte structure and samplings
The four macrophyte species (Chara aculeolata, Stratiotes aloides, Potamogeton lucens and Myriophyllum spicatum) chosen for the analysis of spatial distribution of larval chironomid differ in their morphological structure, and are expected to show differences in colonisation area and potential food source (periphytic algae) for chironomids.
Hedgehog stonewort (Ch. aculeolata) is a highly developed green alga; in the studied lake it forms dense mats covering the sediments. Water soldier (S. aloides) is a vascular plant species forming large rosettes which float on the water surface from late spring to autumn. Shining pondweed (P. lucens) forms long, rooted stems with lanceolate leaves; the plants grow up to the water surface. Eurasian watermilfoil (M. spicatum) is a perennial plant species that forms short, branched shoots with finely dissected leaves.
For the evaluation of the biomass of macrophyte species (g wet weight (WW) m−2), the plants were harvested using a Bernatowicz rake (surface area 0.16 m2) (Bernatowicz 1960); at each sampling date and site three replicates were taken. Each macrophyte sample was put into a separate plastic bag. At the laboratory the plants were washed gently under running tap water to avoid tissue damage, blotted out to remove the surface moisture, and weighed to obtain the WW. Each sample was washed and weighed separately.
Periphytic algae sampling
A sample was taken by cutting off a plant fragment (ensuring the length was the same for all macrophyte species) in a water column, and putting it into a plastic bag. The content of each bag was transferred into a plastic bottle filled with 300 ml of distilled water. Epiphytic algae were separated from the plant by shaking the sealed bottle vigorously for 2 min. The suspension was filtered through a 300-μm mesh size to avoid contamination by small plant fragments or occasional invertebrates. From this sample, a 100-ml algal sample was fixed with Lugolʼs solution and then with a formaldehyde-glycerin solution. A 2-ml aliquot was taken from the subsample and put in a 10-ml Utermöhl counting chamber and then filled with filtered water (GF/C filter). After settling, at least 200 algal cells were counted along one or more grid lines. All cells of colonial algae were counted; each filament with a length of 100 μm was counted as one cell. Counting and identification were performed at 400× magnification under an inverted microscope equipped with a calibration micrometer. Identification to genus level was based on the keys of van den Hoek et al. (1995). The biomass of the periphytic algae was calculated using the lengths and widths of algal cells and common geometric equations (Rott 1981), and expressed in micrograms per 100 g of dry weight (DW) of plant. The plant fragments collected for the analysis were dried at 105 °C for 24 h and then weighed to obtain the DW. The relative abundance of algal taxonomic groups was estimated in the total biomass of algae, based on the biomass of counted cells and filaments.
Chironomid sampling
Chironomid larvae were collected from the periphyton using a perspex cylindrical apparatus (wall thickness 0.5 cm, length 32 cm, diameter 13 cm, weight 800 g), with openings covered by 250-μm mesh nets (Kornijów 1998). The open apparatus was lowered into the macrophyte bed and a plant fragment was put very gently inside the cylinder using a small floristic fork. The sampler was closed very slowly to minimise water movement and raised in a horizontal position to the surface. The shoots protruding beyond the top of the sampler were cut off. The water from the cylinder flowed out, the sampler was opened and the plant material was transferred to a plastic bag. Next, the sampler was put in a vertical position and flushed with water outside to rinse animals remaining on the net to the bottom of the cylinder where they could be collected easily. At the laboratory, the larvae were removed from the macrophyte samples, preserved in 4 % formaldehyde solution, counted and identified. Chironomidae larvae nomenclature followed Chernovski (1949) and Wiederholm (1983). Density was calculated per 100 g DW of plant. The DW of plants collected in a single sample was estimated using the same method as for periphytic algae.
Gut analysis
At each macrophyte species sampling site and on each sampling date (month), ten larvae of each dominant taxon were selected for gut content analysis. The larvae were rinsed of any surface debris, decapitated and dissected along the length of their body and placed in Eppendorf tubes filled with filtered water (GF/C filter). Each tube stored the content of one single gut. The tubes were fixed on a shaker for 20 min to dislodge the gut contents from the digestive tube tissues. Next, the gut content solutions were put separately into a 10-ml counting chamber and filled with filtered water (GF/C). After settling, algae in the gut contents were identified and counted under an inverted microscope.
The relative abundance of cyanobacteria, diatoms, and chlorophytes found in the gut were assessed as a percentage ratio of the total number of cells (filaments) of each algal group to the total number of particles counted on the slide. Each filament of 100 μm length was calculated as one cell.
Physical and chemical water parameters
Water samples for chemical analysis were taken bimonthly from January 2008 to November 2009, simultaneously with periphytic algae and chironomid samples. Samples were taken inside each macrophyte bed. Three replicates were collected on each sampling date and at each site. Temperature, pH, conductivity and dissolved oxygen were recorded in situ using the YSI 556 MPS electrode. Planktonic and periphytic chlorophyll-a values were determined by the spectrophotometric method following a 24-h extraction with 90 % acetone in the dark (Golterman 1969). Concentrations of nutrients (N–NH4, N–NO3, TP, P–PO4) were analysed using spectrophotometric methods (Hermanowicz et al. 1999).
Data analysis
All data collected during the 2-year study was verified statistically. Data was log + 1 transformed to normal distribution. The statistical analysis showed a lack of significant inter-annual differences, thus the results presented in Figures and Tables are mean values for 2008–2009 for each month of collection. The influence of macrophyte species and season on the biomass of periphytic algae and spatial distribution of chironomid larvae was verified using two-way analysis of variance (ANOVA). To recognise the role of periphytic algae as a direct food source for chironomids, Pearsonʼs correlation coefficients were calculated. The correlations were calculated on the data of chironomid abundance and algal biomass and calculations were made using all data collected during the 2-year study. For each macrophyte species site, the number of samples was n = 36 (6 months × 3 replicates × 2 years). Analysis was performed using STATISTICA 7.0 Software (Statsoft Inc., 2004).
Ordination analysis was performed to specify habitat conditions responsible for chironomid distribution (abundance) between plant species. Canonical correspondence analysis (CCA) was used to explore the relationships between the abundance of chironomid taxa and environmental variables (ter Braak and Šmilauer 2002). Automatic forward selection of environmental variables (Monte Carlo permutation test) was used to determine the most important variables (Lepš and Šmilauer 2003). Variables whose level of significance exceeded 0.05 were marked passively on the diagrams. The ordination analyses were performed using CANOCO 4.5 for Windows®.
Results
Physical and chemical parameters of the water
At the studied sites, the physical and chemical water parameter values were typical for slightly eutrophic, macrophyte-dominated lakes. Concentrations of planktonic chlorophyll-a were low (2.9–42.8 μg l−1) and resulted in high Secchi disc transparency (0.5–1.5 m). Concentrations of nitrogen compounds were affected by season; N–NH4 showed the highest values in September (0.552–0.934 mg l−1) and N–NO3 in November (0.436–0.525 mg l−1). Concentrations of TP depended on the site and reached the highest values in May, with 0.116 and 0.059 mg l−1 for Ch. aculeolata and S. aloides, respectively; July with a value of 0.091 mg l−1 for P. lucens; and November, with a value of 0.440 mg l−1 for M. spicatum. The highest concentrations of P–PO4 were noted in January (0.024–0.033 mg l−1), except for the site with P. lucens. At that site, the peak value of P–PO4 (0.043 mg l−1) was found in July.
Structure of biomass of macrophyte species
The mean biomass of the macrophytes studied was in the range of 254.7–2,238.2 g WW m−2 and showed a clear variation between the months (Table 1). On individual macrophyte species, the lowest biomass (254.7–909.2 g WW m−2) was observed in January under ice cover and the highest (1,097.7–2,238.2 g WW−2) mostly in July, the peak of the growing season. P. lucens showed the highest biomass (1,105.6 g WW m−2) in May.
Structure and biomass of periphytic algae
The total biomass of periphytic algae (μg 100 g DW−1) differed significantly between macrophyte species (ANOVA; F = 55.93; P < 0.001) and seasons (ANOVA; F = 50.56; P < 0.001) (Fig. 1). At most sites the highest biomass of periphytic algae (41.9–53.8 μg 100 g DW−1) was observed under ice cover in January, and the lowest in September (4.7–7.3 μg 100 g DW−1). At the Ch. aculeolata site, the highest total algal biomass was recorded in July (25.4 μg 100 g DW−1) and the lowest in November (14.8 μg 100 g DW−1).
Three taxonomic algal groups (chlorophytes, diatoms and cyanobacteria) were observed on the studied macrophytes (Fig. 1). Diatoms were predominant in the algal biomass on Ch. aculeolata (6.2–9.8 μg 100 g DW−1) and M. spicatum (2.5–20.8 μg 100 g DW−1). On S. aloides and P. lucens, diatoms were dominant in January (26.3 and 18.8 μg 100 g DW−1, respectively); March (9.9 and 2.4 μg 100 g DW−1, respectively); and November (10.6 and 5.8 μg 100 g DW−1, respectively). In the remaining months, the highest biomass was noted for chlorophytes (1.9–11.6 μg 100 g DW−1). Cyanobacteria showed the highest biomass in January (9.2–26.3 μg 100 g DW−1) and November (5.8–10.6 μg 100 g DW−1).
Density and dominance of chironomid larvae
The total density of chironomid larvae varied from 9 individuals 100 g DW−1 (September, Ch. aculeolata) to 289 individuals 100 g DW−1 (January, S. aloides) and was significantly affected by the season (ANOVA, F = 1.41; P = 0.027) and macrophyte species (ANOVA, F = 3.19; P = 0.004) (Fig. 2). On P. lucens (25–202 individuals 100 g DW−1) and S. aloides (17–289 individuals 100 g DW−1) chironomids showed visibly higher abundance. The lowest densities were usually noted in summer (July, September) and the highest in winter (January, March) (Fig. 2).
In total, 18 taxa of larval chironomids were noted on the macrophyte species studied (Table 2). There were differences between macrophytes in the number of taxa as well as in their abundance. The highest number of taxa (12) was observed on S. aloides and P. lucens and the lowest (7 taxa) on Ch. aculeolata. On Ch. aculeolata, larvae of Dicrotendipes sp. (22–48 individuals 100 g DW−1) and Paratanytarsus austriacus (11–30 individuals 100 g DW−1) showed the highest abundance. On S. aloides, larvae of Diplocladius cultriger (15–92 individuals 100 g DW−1), Dicrotendipes sp. (5–61 individuals 100 g DW−1) and Glyptotendipes sp. were dominant, with numbers reaching from 8 to 55 individuals 100 g DW−1. Larvae of Dicrotendipes sp. (3–44 individuals 100 g DW−1), Paratanytarsus austriacus (16–186 individuals 100 g DW−1) and Endochironomus albipennis gr. (3–68 individuals 100 g DW−1) were very abundant on Potamogeton lucens. On M. spicatum, the larvae of Psectrocladius sordidellus gr. (37–39 individuals 100 g DW−1), Dicrotendipes sp. (5–38 individuals 100 g DW−1) and Cricotopus sylvestris gr. were dominant, and amounted to 16–20 individuals 100 g DW−1.
Periphytic algae in the diet of chironomids
The relative abundance of periphytic algae in the chironomid gut showed high variability between seasons, macrophyte and chironomid species, and varied from 30 to 90 % (Fig. 3). The remainder of the chironomid diet comprised amorphous detritus. The highest relative abundance of algae was observed in the diet of Dicrotendipes sp. (40–90 %), Glyptotendipes sp. (40–85 %), Endochironomus albipennis (70–78 %) and Endochironomus impar (70–78 %). The larvae fed mostly on diatoms; their percentages varied between 13 and 68 % of the larval diet (Fig. 3). Cyanobacteria were noted in the chironomid diet occasionally, and in low proportions (2–8 %).
Chironomids associated with S. aloides and Potamogeton lucens showed the highest proportion of algae in their guts (Fig. 3). Depending on the season, the relative abundance of algae in the larval diet varied from 30 to 90 % (S. aloides) and from 35 to 78 % (P. lucens). On M. spicatum, the proportions of algae in the chironomid gut were visibly lower. The relative abundance of algae in the larval diet did not usually exceed 50 %.
Environmental conditions versus chironomid structure
The results of CCA for spatial distribution of chironomid larvae showed that all environmental variables accounted for 73.2 % of the total variance of chironomid abundance. However, only four variables (the results of the Monte Carlo permutation test) showed a significant effect on the distribution of chironomid larvae between macrophyte species: temperature (λ = 0.52; F = 3.50; P = 0.002); Secchi disc transparency (λ = 0.37; F = 2.71; P = 0.002); biomass of chlorophytes (λ = 0.34; F = 2.86; P = 0.004); and diatoms (λ = 0.28; F = 2.16; P = 0.010). The CCA plots (Fig. 4a,b) show the samples separated into three groups by macrophyte species: S. aloides (group I), P. lucens (group II), Ch. aculeolata and M. spicatum (group III). The chironomid taxa (Diplocladius cultriger, Dicrotendipes sp., Endochironomus impar, Glyptotendipes sp., Microtendipes rydalensis and Polypedilum nubeculosum gr.) collected on S. aloides correspond with a rising gradient of dissolved oxygen, P–PO4 and diatom biomass. The largest group of chironomid taxa (Psectrocladius sp., Allochironomus sp., Cladotanytarsus mancus, Paratanytarsus austriacus, Endochironomus albipennis gr., Phaenopsectra flavipes, Polypedilum convictum gr., P. sordens) associated with Potamogeton lucens showed a positive relation to the rising temperature gradient, Secchi disc transparency and chlorophyte biomass. The abundance of chironomid taxa Ablabesmyia phatta, Cricotopus sylvestris gr., Psectrocladius sordidellus gr., and Cladopelma lateralis observed on Ch. aculeolata and Myriophyllum spicatum corresponded with higher concentrations of N–NH4, N–NO3, Ptot, conductivity and cyanobacterial biomass.
Relationships between algae and chironomids
A significant relationship between algal biomass and chironomid density was noted only for the most abundant chironomid taxa (Table 3). Chironomid taxa, Diplocladius cultriger, Glyptotendipes sp., Microtendipes rydalensis gr. and Phaenopsectra flavipes showed a significant correlation with periphytic algae on S. aloides. The abundance of Psectrocladius sordidellus gr., Dicrotendipes sp. and Polypedilum sordens was positively related to algal biomass on Potamogeton lucens. The density of Cricotopus sylvestris gr. was positively correlated with algal biomass on Myriophyllum spicatum. A significant relationship between Paratanytarsus austriacus and algae was noted on Ch. aculeolata.
Discussion
The macrophyte species studied differ in terms of total biomass of periphytic algae, but not in the structure of the algal community. Diatoms were the dominant periphytic algae on the studied macrophytes, and such a taxonomic composition is typical for lakes with abundant soft vegetation (Pip and Robinson 1984; Gross et al. 2003). Cyanobacteria were a minor component of the periphyton community. These algae often dominate at low light availability and in nutrient-rich lakes (Loeb and Reuter 1981; Toporowska et al. 2010). Allelopathy may be another explanation of low cyanobacterial biomass on the studied plant species. Excretion of toxic substances from Chara or Myriophyllum may inhibit the growth of algae, and has been suggested as being responsible for the low density of cyanobacteria (Mulderij et al. 2007; Bauer et al. 2009). The converse results were presented in the studies of Moeller et al. (1988) and Kahlert and Pettersson (2002); these demonstrated that under low nutrient availability, the periphyton communities associated with macrophytes are highly host-specific. At the study site, Lake Skomielno, the morphology of plant species does not appear to be the main factor influencing periphyton richness. Conversely, the results of Laugaste and Reunanen (2005) showed that the submerged plants richest in periphytic algae are densely growing and have fine branchlets, such as Ranunculus circinatus Sibth. (Ranun), Myriophyllum verticillatum L., and Potamogeton pectinatus L. The highest algal biomass was observed on Stratiotes aloides, a macrophyte species with a simple morphological structure. Studies provided by Cattaneo (1983) showed that the sword-shaped leaves of S. aloides act as a substrate for intensive algal development and accumulation of detritus, the basic components of the food of epiphytic grazers. The lowest biomass of periphytic algae was observed on Chara aculeolata. This observation concurs with results of the studies of Blindow (1987) as well those of Laugaste and Reunanen (2005). The authors stressed the negative effect of calcite encrusting living charophyte plants on the biomass of periphytic algae. Calcareous deposits on charophytes do not provide a suitable substratum for stalked or adnate diatoms.
In a macrophyte-dominated lake, periphytic algae showed a seasonal pattern of succession: the highest diatom biomass was observed in winter and spring (January and May), while chlorophytes developed more intensively in summer and autumn (July and September) (Meulemans and Roos 1985; Vermaat and Hootsmans 1994). Seasonal changes in the structure and biomass of periphytic algae on the macrophyte species studied will be a result of water temperature and light conditions. Temperature affects enzymatic processes and growth rate. Higher temperatures may favour the development of chlorophytes, and at lower temperatures diatoms are dominant (Vermaat 2005). In the study, diatoms showed a higher biomass in January and March. Apart from temperature, light is a prime abiotic factor affecting the composition of periphytic algae. High daylight intensity during summer may inhibit the growth of diatoms (Albay and Aykulu 2002). Light conditions in the studied lake were not related to water depth. At the study sites, Secchi disc transparency was very high, and with the exception of the winter period, reached the bottom. The possible differences in periphyton biomass between the macrophyte species are probably a consequence of their architecture, leaf type, lifespan of leaves or vertical distribution of their biomass in the water column (Romo and Galanti 1998; Noges et al. 2010). These features affect periphyton-macrophyte competition for light during the vegetation period, and determine the growth and succession of both groups.
Despite the abiotic factors, the composition and biomass of periphytic algae were structured by grazing chironomids. Both the results of CCA and gut content analysis confirmed the significant role of Glyptotendipes sp., Endochironomus albipennis gr., Endochironomus impar and Dicrotendipes sp. as consumers of periphytic diatoms. The results confirm the role of diatoms as a basic component of the chironomid diet, as reported by other studies (Pinder 1992; Tall et al. 2006).
In a macrophyte-dominated lake, larval chironomids showed spatial distribution between macrophyte species. In contrast to the results of studies by Dvorak and Best (1982), Cyr and Downing (1988) and Cheruvelil et al. (2000), the highest taxa richness and density of chironomids were noted on macrophytes with simple morphological structure (leaf architecture): S. aloides and P. lucens. On both macrophytes, surface dwellers and miner species were observed. The density of miner species was very low (about 10 individuals 100 g DW−1). The mining larvae on both macrophyte species were represented mostly by larvae of Glyptotendipes sp., a commonly reported leaf-miner (Kornijów 1986; Tarkowska-Kukuryk 2006). On the leaf surface, Cricotopus sylvestris gr., Psectrocladius sordidellus gr., Dicrotendipes sp., Glyptotendipes sp., Endochironomus albipennis gr. and Paratanytarsus austriacus were dominant. These taxa are typical for eutrophic lakes with well-developed submerged vegetation (Lods-Crozet and Lachavanne 1994; Berg 1995; Cerba et al. 2010). The highest density of chironomids was observed on S. aloides, and the highest species richness on Potamogeton lucens. The chironomid abundance on both macrophytes was positively related to the biomass of periphytic algae and may indicate the direct role of algae as food for grazing larvae. On S. aloides, the highest proportion of algae in the diet was observed in July and September. At that time, the total algal biomass was at its lowest, and larvae of Glyptotendipes sp. predominated among the chironomids. A similar algae-chironomid relationship was noted on P. lucens in March and September. In both seasons, the biomass of algae was very low while its proportion in the diet of dominant chironomids (Endochironomus albipennis gr. and Endochironomus impar) exceeded 70 %. The role of Glyptotendipes sp. and Endochironomus albipennis gr. as important top-regulators of periphytic algal biomass was indicated previously in a shallow, phytoplankton-dominated (turbid water) lake (Tarkowska-Kukuryk 2013). The high proportions of algae in the gut of dominant chironomid taxa suggest that under low nutrient concentrations (a clear-water lake), the availability of food (algae) should be considered as an important determinant of chironomid distribution.
The distribution of chironomids between macrophyte species was affected by environmental factors. CCA analysis separated studied macrophytes and chironomids into three groups. The abundance of chironomid taxa associated with S. aloides was closely related to dissolved oxygen and P–PO4. The presence of Stratiotes decreases concentrations of phosphorus; the plant might withdraw nutrients not only from the sediment but also from the surrounding water (Brammer and Wetzel 1984). Precipitation of phosphorus on Stratiotes leaves can be a possible explanation for clear water (high oxygen content) around Stratiotes plants. Temperature (season) and Secchi disc visibility affect the distribution of chironomids on P. lucens. Temperature is regarded as an important abiotic factor regulating the life cycle of chironomids, egg development and emergence of larvae (Gresens 1997). The Secchi disc depth may help to explain the role of light in the production of different parts of the macrophyte; generally, the upper part has greater light availability because of better exposure (Wetzel 2001). Light availability can control vertical distribution of periphyton on leaves of Potamogeton and indirectly affects its colonisation by chironomids. The analysis of algae-chironomid relations on Ch. aculeolata and M. spicatum indicates that these macrophytes create similar, less favourable habitats for periphytic algae and associated chironomids. Charophytes concentrate most of their biomass close to the sediment surface, and at that depth the development of periphytic algae is light limited (van den Berg et al. 1998). Low availability of algae (food) does not create a favourable substrate for chironomids. On M. spicatum, the chironomid structure was specific; larvae of Cricotopus sylvestris gr. were only noted at the site. It has been reported that larvae of the genus Cricotopus are very host-specific to Myriophyllum species (Menzie 1981; Balci and Kennedy 2003). The larvae are able to feed on meristematic tissue, suppressing the plantʼs growth (McRae et al. 1990). Moreover, CCA analysis showed the relationship between chironomid assemblages on Ch. aculeolata and M. spicatum to the concentrations of N–NH4 and N–NO3. The concentration of nitrogen compounds may have a significant effect on the increase in length of Chara spp. (Simons et al. 1994), as well the increase in biomass of M. spicatum (Anderson and Kalff 1986). The algal population (food source) in macrophyte-dominated shallow lakes is often found to be nitrogen limited (Moss et al. 2005).
The habitat (macrophyte species) preferences of larval chironomids in a shallow clear-water lake are related to the biomass of periphytic algae on host plants and to environmental variables (oxygen, nutrients) specific to the macrophyte stand.
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Acknowledgments
The study was supported by the research grant N N304 3391 33 “Trophic interactions between macrophytes, periphytic algae and epiphytic invertebrates in shallow lake ecosystems”, provided by the Polish National Science Foundation. I would like to thank three anonymous reviewers for many valuable critical comments to the manuscript.
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Tarkowska-Kukuryk, M. Spatial distribution of epiphytic chironomid larvae in a shallow macrophyte-dominated lake: effect of macrophyte species and food resources. Limnology 15, 141–153 (2014). https://doi.org/10.1007/s10201-014-0425-4
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DOI: https://doi.org/10.1007/s10201-014-0425-4