Skip to main content
SpringerLink
Log in

Morphological and molecular characterization of a natural hybrid betweenOrchis laxiflora andO. morio (Orchidaceae)

  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

A putative natural hybrid betweenOrchis laxiflora andO. morio (Orchidaceae) from southern Italy, formerly known asO. alata, was characterized both on morphological and molecular grounds in order to confirm its hybrid status and to trace its maternal lineage. The morphological characters of the putative hybrid showed intermediacy between those of the parent species, and restriction fragment length polymorphism (RFLP) analysis of a region of the nuclear ribosomal DNA confirmed its hybrid origin. Chloroplast DNA RFLP analysis indicated thatO. morio provided the maternal genome.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arnold, M. L., Bennet, B. D., Zimmer, E. A., 1990: Natural hybridization betweenIris fulva andIris hexagona: pattern of ribosomal DNA evolution. — Evolution44: 1512–1521.

    Google Scholar 

  • Bianco, P., D'Emerico, S., Medagli, P., Ruggiero, L., 1990: ×Orchiserapias nelsoniana Bianco, D'Emerico, Medagli etRuggiero, hybr. nat. nov. della Puglia. — Webbia44: 315–322.

    Google Scholar 

  • Caputo, P., Stevenson, D. W., Wurtzel, E. T., 1991: A phylogenetic analysis of American Cycads (Cycadales) using chloroplast DNA restriction fragment polymorphism. — Brittonia43: 135–145.

    Google Scholar 

  • Corriveau, J. L., Coleman, A. W., 1988: Rapid screening method to detect potential biparental inheritance of plastid DNA and results for over 200 angiosperm species. — Amer. J. Bot.75: 1443–1458.

    Google Scholar 

  • Cozzolino, S., Aceto, S., 1995 “1994”: Morphological and molecular characterization of ×Orchiaceras bergonii (Nanteuil)E. G. Cam. — Giorn. Bot. Ital.128: 861–867.

    Google Scholar 

  • Danesch, O., Danesch, E., 1972: Orchideen Europas,Ophrys-Hybriden. — Bern, Stuttgart: Hallwag.

    Google Scholar 

  • —, —,Ehrendorfer, F., Ehrendorfer, K., 1975: Hybriden und hybridogene Sippen ausOphrys bertolonii andOphrys atrata (Orchidaceae). — Pl. Syst. Evol.124: 79–123.

    Google Scholar 

  • Derbyshire, S. J., Cayouette, J., Warwick, S. I., 1992: The intergeneric hybrid origin ofPoa labradorica (Poaceae). — Pl. Syst. Evol.181: 57–76.

    Google Scholar 

  • Doyle, J. J., Doyle, J. L., 1988: Natural interspecific hybridization in Eastern North AmericanClaytonia. — Amer. J. Bot.75: 1238–1246.

    Google Scholar 

  • —, 1989: 5S nuclear ribosomal gene variation in theGlycine tomentella polyploid complex. — Syst. Bot.14: 621–624.

    Google Scholar 

  • Ehrendorfer, F., 1980: Hybridisierung, Polyploidie und Evolution bei europäisch-mediterranen Orchideen. — Jahresber. Naturwiss. Vereins Wuppertal33: 15–34.

    Google Scholar 

  • Feinberg, A. P., Vogelstein, B., 1983: A technique for radiolabelling DNA restriction endonucleases fragments to high specific activiy. — Analytical Biochem.132: 6–13.

    Google Scholar 

  • Fleury, J. F., 1819: Orchidées des environs de Rennes. — Rennes.

  • Gillot, M. X., 1881: Note sur l'Orchis alata Fleury. — Bull. Soc. Bot. France28: 307–320.

    Google Scholar 

  • —, 1897:Orchis alata Fleury. Morphologie et anatomie. — Monde Pl. Rev. Mens. Bot.7: 93–97.

    Google Scholar 

  • Gottlieb, L. D., 1972: Levels of confidence in the analysis of hybridization in plants. — Ann. Missouri Bot. Gard.59: 435–446.

    Google Scholar 

  • Grant, V., 1981: Plant speciation. — New York: Columbia University Press.

    Google Scholar 

  • Greilhuber, J., Ehrendorfer, F., 1975: Chromosome numbers and evolution inOphrys (Orchidaceae). — Pl. Syst. Evol.124: 125–138.

    Google Scholar 

  • Harris, S.A., Ingram, R., 1992: Chloroplast DNA and biosystematics: the effect of intraspecific diversity and plastid transmission. — Taxon40: 393–412.

    Google Scholar 

  • Hughes, C. E., Harris, S. A., 1994: The characterisation and identification of a naturally occurring hybrid in the genusLeucaena (Leguminosae:Mimosoideae). — Pl. Syst. Evol.192: 177–197.

    Google Scholar 

  • Jorgensen, R. A., Cluster, P. D., 1988: Modes and tempos in the evolution of nuclear ribosomal DNA: new characters for evolutionary studies and new markers for genetic and population studies. — Ann. Missouri Bot. Gard.75: 1238–1247.

    Google Scholar 

  • Lassimonne, S. E., 1897: ×Orchis alata et ×O. alatiflora. — Monde Pl. Rev. Mens. Bot.7: 131.

    Google Scholar 

  • Nazzaro, R., Menale, B., Di Novella, N., 1995: LeOrchidaceae della zona occidentale del Vallo di Diano (Salerno). — Webbia50: 25–35.

    Google Scholar 

  • Palmer, J. D., 1985: Isolation and structural analysis of chloroplast DNA. — Meth. Enzymol.118: 167–186.

    Google Scholar 

  • Reichenbach, H. G., 1850–51:Orchideae in Flora Germanica recensitae. —Reichenbach, H. G. L.: Icones Flora Germanicae et Helveticae,13 &14. — Leipzig: Hofmeister.

    Google Scholar 

  • Reuter, G.F., 1861: Catalogue des plantes vasculaires qui croissent naturellement aux environs de Genève. 2nd edn. — Genève: Kessmann.

    Google Scholar 

  • Rieseberg, L. H., Brunsfield, S.J., 1992: Molecular evidence and plant introgression. — InSoltis, P. S., Soltis, D. E., Doyle, J. J., (Eds): Molecular systematics of plants, pp. 151–176. — London: Chapman & Hall.

    Google Scholar 

  • —, 1990: Molecular test of the hypothesized hybrid origin of two diploidHelianthus species (Asteraceae). — Evolution44: 1498–1511.

    Google Scholar 

  • Rogers, S. Q., Bendich, A. J., 1987: Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. — Pl. Molec. Biol.9: 509–520.

    Google Scholar 

  • Rossi, W., Arduino, P., Cianchi, R., Bullini, L., 1992: A new natural hybrid in the genusOrchis L.: genetic data and description. — Lindleyana7: 121–126.

    Google Scholar 

  • Sambrook, J., Fritsch, E. F., Maniatis, T., 1989: Molecular cloning. A laboratory manual. 2nd edn. — Cold Spring Harbor: CSH Press.

    Google Scholar 

  • Soltis, P. S., Soltis, D. E., 1991: Multiple origin of the allotetraploidTragopogon minus (Compositae): rDNA evidence. — Syst. Bot.16: 407–413.

    Google Scholar 

  • Stace, C. A., 1975: Hybridization and the flora of the British Isles. — London: Academic Press.

    Google Scholar 

  • Stebbins, G. L., 1959: The role of hybridization in evolution. — Proc. Amer. Philos. Soc.103: 231–251.

    Google Scholar 

  • —, 1956: Population variability, hybridization, and introgression in some species ofOphrys. — Evolution10: 32–46.

    Google Scholar 

  • Steinbrück, G., Schlegel, M., Dahlström, I., Röttger, B., 1986: Characterization of interspecific hybrids betweenOrchis mascula andO. pallens (Orchidaceae) by enzyme electrophoresis. — Pl. Syst. Evol.153: 229–241.

    Google Scholar 

  • Sugiura, M., Shinozaki, K., Zaita, N., Kusuda, M., Kumano, M., 1986: Clone bank of the tobacco (Nicotiana tabacum) chloroplast genome as a set of overlapping restriction endonuclease fragments: mapping of eleven ribosomal protein genes. — Pl. Sci.44: 211–216.

    Google Scholar 

  • Sundermann, H., 1980: Europäische und mediterrane Orchideen. — Hildesheim: Brücke.

    Google Scholar 

  • Sytsma, K. J., Gottlieb, L.D., 1986: Chloroplast DNA evolution and phylogenetic relationships inClarkia sect.Peripetasma (Onagraceae). — Evolution40: 1248–1261.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dipartimento di Biologia Vegetale, Universitá di Napoli “Federico II”, Via Foria, 223, I-80139, Napoli, Italy

    P. Caputo

  2. Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli “Federico II”, Via Mezzocannone, 8, I-80134, Napoli, Italy

    S. Aceto

  3. Orto Botanico, Università di Napoli “Federico II”, Via Foria, 223, I-80139, Napoli, Italy

    S. Cozzolino

  4. Dipartimento di Biologia Vegetale, Universitá di Napoli “Federico II”, Via Foria, 223, I-80139, Napoli, Italy

    R. Nazzaro

Authors
  1. P. Caputo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Aceto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Cozzolino
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. Nazzaro
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Caputo, P., Aceto, S., Cozzolino, S. et al. Morphological and molecular characterization of a natural hybrid betweenOrchis laxiflora andO. morio (Orchidaceae). Pl Syst Evol 205, 147–155 (1997). https://doi.org/10.1007/BF01464401

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01464401

Key words

Search

Navigation