Fig 1.
Sequential FISH and GISH patterns of Elymus sibiricus ‘Nongmu’.
a: FISH pattern probed with (AAG)10 (green) and Oligo-pAs1 (red); b: Sequential GISH pattern probed with Hordeum bogdanii (green) and Pseudoroegneria stipifolia (red). Scale bar = 10 μm.
Fig 2.
Molecular karyotyping of the two cultivars of Elymus sibiricus combined with Oligo-pAs1 (red) and different repetitive sequences (green). The upper panel shows the karyotype for Tongde and the lower for Nongmu, for each characterization.
Fig 3.
Karyotyping of four Elymus sibiricus cultivars, probed with Oligo-pSc119.2 (green), (AAG)10 (yellow), and Oligo-pAs1 (red). A1 and A2: Two different samples from Tongde; B: Chuancao No. 2; C: Qingmu No. 1; and D: Nongmu.
Fig 4.
Ideogram of FISH-banded chromosomes of Elymus sibiricus.
The black star next to the FISH signal indicates the polymorphisms.